Noi:Sample tracking: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Noi
No edit summary
>Noi
No edit summary
Line 1: Line 1:
=November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)=
=November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)=
- received 8 gDNA samples from Paula Ladd<br>
- received 8 gDNA samples from Paula Ladd<br>
Note: WT: wildtype, TG: transgenic
 
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''Samples'''
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''concentration (ng/ul)'''
| align="center" style="background:#f0f0f0;"|'''volume (ul)'''
|-
| 1. wild-type cortex||WT-CTX||2000.0||3.8
|-
| 2. wild-type cerebellum||WT-CBL||100.0||8.2
|-
| 3. wild-type brain stem||WT-BS||600.0||8.5
|-
| 4. wild-type retina||WT-RET||1800.0||4.0
|-
| 5. transgenic cortex||TG-CTX||250.0||17.0
|-
| 6. transgenic cerebellum||TG-CBL||400.0||14.0
|-
| 7. transgenic brain stem||TG-BS||1000.0||6.5
|-
| 8. transgenic retina||TG-RET||2000.0||8.0
|}
Note: WT: wild-type, TG: transgenic
Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''Samples'''
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''total volume (ul) after diluted'''
| align="center" style="background:#f0f0f0;"|'''concentration(ng/ul)'''
|-
| 1. wild-type cortex||WT-CTX||25.0||223.2
|-
| 2. wild-type cerebellum||WT-CBL||25.0||663.5
|-
| 3. wild-type brain stem||WT-BS||25.0||86.0
|-
| 4. wild-type retina||WT-RET||25.0||163.7
|-
| 5. transgenic cortex||TG-CTX||20.0||219.2
|-
| 6. transgenic cerebellum||TG-CBL||25.0||109.4
|-
| 7. transgenic brain stem||TG-BS||25.0||103.0
|-
| 8. transgenic retina||TG-RET||25.0||126.6
|}<br> labnote: 2010_11_08





Revision as of 07:51, 10 December 2010

November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)

- received 8 gDNA samples from Paula Ladd

Samples Sample ID concentration (ng/ul) volume (ul)
1. wild-type cortex WT-CTX 2000.0 3.8
2. wild-type cerebellum WT-CBL 100.0 8.2
3. wild-type brain stem WT-BS 600.0 8.5
4. wild-type retina WT-RET 1800.0 4.0
5. transgenic cortex TG-CTX 250.0 17.0
6. transgenic cerebellum TG-CBL 400.0 14.0
7. transgenic brain stem TG-BS 1000.0 6.5
8. transgenic retina TG-RET 2000.0 8.0
Note: WT: wild-type, TG: transgenic
Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
Samples Sample ID total volume (ul) after diluted concentration(ng/ul)
1. wild-type cortex WT-CTX 25.0 223.2
2. wild-type cerebellum WT-CBL 25.0 663.5
3. wild-type brain stem WT-BS 25.0 86.0
4. wild-type retina WT-RET 25.0 163.7
5. transgenic cortex TG-CTX 20.0 219.2
6. transgenic cerebellum TG-CBL 25.0 109.4
7. transgenic brain stem TG-BS 25.0 103.0
8. transgenic retina TG-RET 25.0 126.6


labnote: 2010_11_08


December 7, 2010 (Sarah's African gDNA, UPenn )

- received 48 methylome samples
William R Beggs, Jr.
Tishkoff Lab - Dept of Genetics, University of Pennsylvania
430 Clinical Research Building
415 Curie Blvd
Philadelphia, PA 19104-6145

LabID 1M sample # Well Volume ul Conc ng/ul approx
CAPB016 A01 50 100
CAPB046 B01 50 100
CAPB056a C01 50 100
CAPL056 D01 50 100
CAPM007 E01 50 100
TZHZ018A F01 50 100
TZHZ033B G01 50 100
TZHZ075A H01 50 100
TZHZ214 A02 50 100
TZHZ221B B02 50 100
TZSW067 C02 50 100
TZSW128B D02 50 100
TZSW131A E02 50 100
TZSW132B F02 50 100
TZSW135B G02 50 100
CAMF013A H02 50 100
CAFU043 A03 50 100
CAFU042 B03 50 100
CAFU028 C03 50 100
CAMF022A D03 50 100
CAPB043 E03 50 100
CAPL049 F03 50 100
CAPM001A G03 50 100
CAPL036 H03 50 100
KEBR007A A04 50 100
KEBR028A B04 50 100
KEBR042B C04 50 100
KEBR040A D04 50 100
KEBR061A E04 50 100
KEPK003A F04 50 100
KEPK006A G04 50 100
KEPK007A H04 50 100
KEPK010A A05 50 100
KEPK016A B05 50 100
CAPM047 son C05 50 100
CAPM003A parent D05 50 100
CAPM004A parent E05 50 100
CAPM056A daughter F05 50 100
ETAM042 G05 50 100
ETAM071 H05 50 100
ETAM077 A06 50 100
ETAM058 B06 50 100
ETAM065 C06 50 100
ETSB008 D06 50 100
ETSB027 E06 50 100
ETSB031 F06 50 100
ETSB035 G06 50 100
ETSB036 H06 50 100

- aliquot 21ul out of 50ul samples into another 96-well plate