Noi:Sample tracking: Difference between revisions
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=November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)= | =November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)= | ||
- received 8 gDNA samples from Paula Ladd<br> | - received 8 gDNA samples from Paula Ladd<br> | ||
Note: WT: | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''concentration (ng/ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''volume (ul)''' | |||
|- | |||
| 1. wild-type cortex||WT-CTX||2000.0||3.8 | |||
|- | |||
| 2. wild-type cerebellum||WT-CBL||100.0||8.2 | |||
|- | |||
| 3. wild-type brain stem||WT-BS||600.0||8.5 | |||
|- | |||
| 4. wild-type retina||WT-RET||1800.0||4.0 | |||
|- | |||
| 5. transgenic cortex||TG-CTX||250.0||17.0 | |||
|- | |||
| 6. transgenic cerebellum||TG-CBL||400.0||14.0 | |||
|- | |||
| 7. transgenic brain stem||TG-BS||1000.0||6.5 | |||
|- | |||
| 8. transgenic retina||TG-RET||2000.0||8.0 | |||
|} | |||
Note: WT: wild-type, TG: transgenic | |||
Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''total volume (ul) after diluted''' | |||
| align="center" style="background:#f0f0f0;"|'''concentration(ng/ul)''' | |||
|- | |||
| 1. wild-type cortex||WT-CTX||25.0||223.2 | |||
|- | |||
| 2. wild-type cerebellum||WT-CBL||25.0||663.5 | |||
|- | |||
| 3. wild-type brain stem||WT-BS||25.0||86.0 | |||
|- | |||
| 4. wild-type retina||WT-RET||25.0||163.7 | |||
|- | |||
| 5. transgenic cortex||TG-CTX||20.0||219.2 | |||
|- | |||
| 6. transgenic cerebellum||TG-CBL||25.0||109.4 | |||
|- | |||
| 7. transgenic brain stem||TG-BS||25.0||103.0 | |||
|- | |||
| 8. transgenic retina||TG-RET||25.0||126.6 | |||
|}<br> labnote: 2010_11_08 | |||
Revision as of 07:51, 10 December 2010
November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)
- received 8 gDNA samples from Paula Ladd
Samples | Sample ID | concentration (ng/ul) | volume (ul) |
1. wild-type cortex | WT-CTX | 2000.0 | 3.8 |
2. wild-type cerebellum | WT-CBL | 100.0 | 8.2 |
3. wild-type brain stem | WT-BS | 600.0 | 8.5 |
4. wild-type retina | WT-RET | 1800.0 | 4.0 |
5. transgenic cortex | TG-CTX | 250.0 | 17.0 |
6. transgenic cerebellum | TG-CBL | 400.0 | 14.0 |
7. transgenic brain stem | TG-BS | 1000.0 | 6.5 |
8. transgenic retina | TG-RET | 2000.0 | 8.0 |
Note: WT: wild-type, TG: transgenic Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
Samples | Sample ID | total volume (ul) after diluted | concentration(ng/ul) |
1. wild-type cortex | WT-CTX | 25.0 | 223.2 |
2. wild-type cerebellum | WT-CBL | 25.0 | 663.5 |
3. wild-type brain stem | WT-BS | 25.0 | 86.0 |
4. wild-type retina | WT-RET | 25.0 | 163.7 |
5. transgenic cortex | TG-CTX | 20.0 | 219.2 |
6. transgenic cerebellum | TG-CBL | 25.0 | 109.4 |
7. transgenic brain stem | TG-BS | 25.0 | 103.0 |
8. transgenic retina | TG-RET | 25.0 | 126.6 |
labnote: 2010_11_08
December 7, 2010 (Sarah's African gDNA, UPenn )
- received 48 methylome samples
William R Beggs, Jr.
Tishkoff Lab - Dept of Genetics, University of Pennsylvania
430 Clinical Research Building
415 Curie Blvd
Philadelphia, PA 19104-6145
LabID | 1M sample # | Well | Volume ul | Conc ng/ul approx |
CAPB016 | A01 | 50 | 100 | |
CAPB046 | B01 | 50 | 100 | |
CAPB056a | C01 | 50 | 100 | |
CAPL056 | D01 | 50 | 100 | |
CAPM007 | E01 | 50 | 100 | |
TZHZ018A | F01 | 50 | 100 | |
TZHZ033B | G01 | 50 | 100 | |
TZHZ075A | H01 | 50 | 100 | |
TZHZ214 | A02 | 50 | 100 | |
TZHZ221B | B02 | 50 | 100 | |
TZSW067 | C02 | 50 | 100 | |
TZSW128B | D02 | 50 | 100 | |
TZSW131A | E02 | 50 | 100 | |
TZSW132B | F02 | 50 | 100 | |
TZSW135B | G02 | 50 | 100 | |
CAMF013A | H02 | 50 | 100 | |
CAFU043 | A03 | 50 | 100 | |
CAFU042 | B03 | 50 | 100 | |
CAFU028 | C03 | 50 | 100 | |
CAMF022A | D03 | 50 | 100 | |
CAPB043 | E03 | 50 | 100 | |
CAPL049 | F03 | 50 | 100 | |
CAPM001A | G03 | 50 | 100 | |
CAPL036 | H03 | 50 | 100 | |
KEBR007A | A04 | 50 | 100 | |
KEBR028A | B04 | 50 | 100 | |
KEBR042B | C04 | 50 | 100 | |
KEBR040A | D04 | 50 | 100 | |
KEBR061A | E04 | 50 | 100 | |
KEPK003A | F04 | 50 | 100 | |
KEPK006A | G04 | 50 | 100 | |
KEPK007A | H04 | 50 | 100 | |
KEPK010A | A05 | 50 | 100 | |
KEPK016A | B05 | 50 | 100 | |
CAPM047 | son | C05 | 50 | 100 |
CAPM003A | parent | D05 | 50 | 100 |
CAPM004A | parent | E05 | 50 | 100 |
CAPM056A | daughter | F05 | 50 | 100 |
ETAM042 | G05 | 50 | 100 | |
ETAM071 | H05 | 50 | 100 | |
ETAM077 | A06 | 50 | 100 | |
ETAM058 | B06 | 50 | 100 | |
ETAM065 | C06 | 50 | 100 | |
ETSB008 | D06 | 50 | 100 | |
ETSB027 | E06 | 50 | 100 | |
ETSB031 | F06 | 50 | 100 | |
ETSB035 | G06 | 50 | 100 | |
ETSB036 | H06 | 50 | 100 |
- aliquot 21ul out of 50ul samples into another 96-well plate