Noi:Sample tracking: Difference between revisions
Jump to navigation
Jump to search
>Noi No edit summary |
>Noi No edit summary |
||
Line 51: | Line 51: | ||
=December 7, 2010 (Sarah's African gDNA, UPenn )= | =December 7, 2010 (Sarah's African gDNA, UPenn )= | ||
- received 48 methylome samples <br>William R Beggs, Jr.<br>Tishkoff Lab - Dept of Genetics, University of Pennsylvania<br>430 Clinical Research Building<br>415 Curie Blvd<br>Philadelphia, PA 19104-6145 | - received 48 methylome samples from Tishkoff's lab delivered by UPS<br> | ||
William R Beggs, Jr.<br>Tishkoff Lab - Dept of Genetics, University of Pennsylvania<br>430 Clinical Research Building<br>415 Curie Blvd<br>Philadelphia, PA 19104-6145 | |||
{| {{table}} border = 1 | {| {{table}} border = 1 | ||
Line 158: | Line 159: | ||
- aliquot 21ul out of 50ul samples into another 96-well plate | - aliquot 21ul out of 50ul samples into another 96-well plate | ||
=December 10, 2010 (normal/tumor gDNA from JHU)= | |||
-received 48 normal/tumor gDNA samples from JHU delivered by FedEx<br> | |||
Eirikur Briem<br> John Hopkins University<br> 855N. Wolfe Street<br> Rangos 580.14<br> Baltimore, MD 21205 | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Sample_ID''' | |||
| align="center" style="background:#f0f0f0;"|'''Kun_Well''' | |||
| align="center" style="background:#f0f0f0;"|'''Final Volume (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final_Conc (ng/uL)''' | |||
|- | |||
| 395||G3||50||30 | |||
|- | |||
| 970||H4||50||30 | |||
|- | |||
| 12||A1||50||30 | |||
|- | |||
| 258||F2||50||30 | |||
|- | |||
| 299||A3||50||30 | |||
|- | |||
| 335||C3||50||30 | |||
|- | |||
| 440||A5||50||30 | |||
|- | |||
| 503||E5||50||30 | |||
|- | |||
| 736||G6||50||30 | |||
|- | |||
| 895||F4||50||30 | |||
|- | |||
| 84||E1||50||30 | |||
|- | |||
| 198||H1||50||30 | |||
|- | |||
| 203||B2||50||30 | |||
|- | |||
| 368||E3||50||30 | |||
|- | |||
| 388||F3||50||30 | |||
|- | |||
| 436||H3||50||30 | |||
|- | |||
| 524||F5||50||30 | |||
|- | |||
| 686||F6||50||30 | |||
|- | |||
| 48||C1||50||30 | |||
|- | |||
| 85||F1||50||30 | |||
|- | |||
| 107||G1||50||30 | |||
|- | |||
| 253||D2||50||30 | |||
|- | |||
| 467||D5||50||30 | |||
|- | |||
| 569||H5||50||30 | |||
|- | |||
| 850||B4||50||30 | |||
|- | |||
| 894||E4||50||30 | |||
|- | |||
| 893||D4||30||50 | |||
|- | |||
| 659||E6||30||50 | |||
|- | |||
| 538||G5||30||50 | |||
|- | |||
| 967||G4||30||50 | |||
|- | |||
| 304||B3||30||50 | |||
|- | |||
| 256||E2||30||50 | |||
|- | |||
| 42||B1||30||50 | |||
|- | |||
| 77||D1||30||50 | |||
|- | |||
| 201||A2||30||50 | |||
|- | |||
| 222||C2||30||50 | |||
|- | |||
| 280||G2||30||50 | |||
|- | |||
| 282||H2||30||50 | |||
|- | |||
| 362||D3||30||50 | |||
|- | |||
| 445||B5||30||50 | |||
|- | |||
| 455||C5||30||50 | |||
|- | |||
| 572||A6||30||50 | |||
|- | |||
| 607||B6||30||50 | |||
|- | |||
| 619||C6||30||50 | |||
|- | |||
| 621||D6||30||50 | |||
|- | |||
| 743||H6||30||50 | |||
|- | |||
| 791||A4||30||50 | |||
|- | |||
| 881||C4||30||50 | |||
|} | |||
sort sample ID into plate layout | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Plate layout''' | |||
| align="center" style="background:#f0f0f0;"|'''1''' | |||
| align="center" style="background:#f0f0f0;"|'''2''' | |||
| align="center" style="background:#f0f0f0;"|'''3''' | |||
| align="center" style="background:#f0f0f0;"|'''4''' | |||
| align="center" style="background:#f0f0f0;"|'''5''' | |||
| align="center" style="background:#f0f0f0;"|'''6''' | |||
|- | |||
| A||12||201||299||791||440||572 | |||
|- | |||
| B||42||203||304||850||445||607 | |||
|- | |||
| C||48||222||335||881||455||619 | |||
|- | |||
| D||77||253||362||893||467||621 | |||
|- | |||
| E||84||256||368||894||503||659 | |||
|- | |||
| F||85||258||388||895||524||686 | |||
|- | |||
| G||107||280||395||967||538||736 | |||
|- | |||
| H||198||282||436||970||569||743 | |||
|} |
Revision as of 20:20, 10 December 2010
November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)
- received 8 gDNA samples from Paula Ladd
Samples | Sample ID | concentration (ng/ul) | volume (ul) |
1. wild-type cortex | WT-CTX | 2000.0 | 3.8 |
2. wild-type cerebellum | WT-CBL | 100.0 | 8.2 |
3. wild-type brain stem | WT-BS | 600.0 | 8.5 |
4. wild-type retina | WT-RET | 1800.0 | 4.0 |
5. transgenic cortex | TG-CTX | 250.0 | 17.0 |
6. transgenic cerebellum | TG-CBL | 400.0 | 14.0 |
7. transgenic brain stem | TG-BS | 1000.0 | 6.5 |
8. transgenic retina | TG-RET | 2000.0 | 8.0 |
Note: WT: wild-type, TG: transgenic Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
Samples | Sample ID | total volume (ul) after diluted | concentration(ng/ul) |
1. wild-type cortex | WT-CTX | 25.0 | 223.2 |
2. wild-type cerebellum | WT-CBL | 25.0 | 663.5 |
3. wild-type brain stem | WT-BS | 25.0 | 86.0 |
4. wild-type retina | WT-RET | 25.0 | 163.7 |
5. transgenic cortex | TG-CTX | 20.0 | 219.2 |
6. transgenic cerebellum | TG-CBL | 25.0 | 109.4 |
7. transgenic brain stem | TG-BS | 25.0 | 103.0 |
8. transgenic retina | TG-RET | 25.0 | 126.6 |
labnote: 2010_11_08
December 7, 2010 (Sarah's African gDNA, UPenn )
- received 48 methylome samples from Tishkoff's lab delivered by UPS
William R Beggs, Jr.
Tishkoff Lab - Dept of Genetics, University of Pennsylvania
430 Clinical Research Building
415 Curie Blvd
Philadelphia, PA 19104-6145
LabID | 1M sample # | Well | Volume ul | Conc ng/ul approx |
CAPB016 | A01 | 50 | 100 | |
CAPB046 | B01 | 50 | 100 | |
CAPB056a | C01 | 50 | 100 | |
CAPL056 | D01 | 50 | 100 | |
CAPM007 | E01 | 50 | 100 | |
TZHZ018A | F01 | 50 | 100 | |
TZHZ033B | G01 | 50 | 100 | |
TZHZ075A | H01 | 50 | 100 | |
TZHZ214 | A02 | 50 | 100 | |
TZHZ221B | B02 | 50 | 100 | |
TZSW067 | C02 | 50 | 100 | |
TZSW128B | D02 | 50 | 100 | |
TZSW131A | E02 | 50 | 100 | |
TZSW132B | F02 | 50 | 100 | |
TZSW135B | G02 | 50 | 100 | |
CAMF013A | H02 | 50 | 100 | |
CAFU043 | A03 | 50 | 100 | |
CAFU042 | B03 | 50 | 100 | |
CAFU028 | C03 | 50 | 100 | |
CAMF022A | D03 | 50 | 100 | |
CAPB043 | E03 | 50 | 100 | |
CAPL049 | F03 | 50 | 100 | |
CAPM001A | G03 | 50 | 100 | |
CAPL036 | H03 | 50 | 100 | |
KEBR007A | A04 | 50 | 100 | |
KEBR028A | B04 | 50 | 100 | |
KEBR042B | C04 | 50 | 100 | |
KEBR040A | D04 | 50 | 100 | |
KEBR061A | E04 | 50 | 100 | |
KEPK003A | F04 | 50 | 100 | |
KEPK006A | G04 | 50 | 100 | |
KEPK007A | H04 | 50 | 100 | |
KEPK010A | A05 | 50 | 100 | |
KEPK016A | B05 | 50 | 100 | |
CAPM047 | son | C05 | 50 | 100 |
CAPM003A | parent | D05 | 50 | 100 |
CAPM004A | parent | E05 | 50 | 100 |
CAPM056A | daughter | F05 | 50 | 100 |
ETAM042 | G05 | 50 | 100 | |
ETAM071 | H05 | 50 | 100 | |
ETAM077 | A06 | 50 | 100 | |
ETAM058 | B06 | 50 | 100 | |
ETAM065 | C06 | 50 | 100 | |
ETSB008 | D06 | 50 | 100 | |
ETSB027 | E06 | 50 | 100 | |
ETSB031 | F06 | 50 | 100 | |
ETSB035 | G06 | 50 | 100 | |
ETSB036 | H06 | 50 | 100 |
- aliquot 21ul out of 50ul samples into another 96-well plate
December 10, 2010 (normal/tumor gDNA from JHU)
-received 48 normal/tumor gDNA samples from JHU delivered by FedEx
Eirikur Briem
John Hopkins University
855N. Wolfe Street
Rangos 580.14
Baltimore, MD 21205
Sample_ID | Kun_Well | Final Volume (uL) | Final_Conc (ng/uL) |
395 | G3 | 50 | 30 |
970 | H4 | 50 | 30 |
12 | A1 | 50 | 30 |
258 | F2 | 50 | 30 |
299 | A3 | 50 | 30 |
335 | C3 | 50 | 30 |
440 | A5 | 50 | 30 |
503 | E5 | 50 | 30 |
736 | G6 | 50 | 30 |
895 | F4 | 50 | 30 |
84 | E1 | 50 | 30 |
198 | H1 | 50 | 30 |
203 | B2 | 50 | 30 |
368 | E3 | 50 | 30 |
388 | F3 | 50 | 30 |
436 | H3 | 50 | 30 |
524 | F5 | 50 | 30 |
686 | F6 | 50 | 30 |
48 | C1 | 50 | 30 |
85 | F1 | 50 | 30 |
107 | G1 | 50 | 30 |
253 | D2 | 50 | 30 |
467 | D5 | 50 | 30 |
569 | H5 | 50 | 30 |
850 | B4 | 50 | 30 |
894 | E4 | 50 | 30 |
893 | D4 | 30 | 50 |
659 | E6 | 30 | 50 |
538 | G5 | 30 | 50 |
967 | G4 | 30 | 50 |
304 | B3 | 30 | 50 |
256 | E2 | 30 | 50 |
42 | B1 | 30 | 50 |
77 | D1 | 30 | 50 |
201 | A2 | 30 | 50 |
222 | C2 | 30 | 50 |
280 | G2 | 30 | 50 |
282 | H2 | 30 | 50 |
362 | D3 | 30 | 50 |
445 | B5 | 30 | 50 |
455 | C5 | 30 | 50 |
572 | A6 | 30 | 50 |
607 | B6 | 30 | 50 |
619 | C6 | 30 | 50 |
621 | D6 | 30 | 50 |
743 | H6 | 30 | 50 |
791 | A4 | 30 | 50 |
881 | C4 | 30 | 50 |
sort sample ID into plate layout
Plate layout | 1 | 2 | 3 | 4 | 5 | 6 |
A | 12 | 201 | 299 | 791 | 440 | 572 |
B | 42 | 203 | 304 | 850 | 445 | 607 |
C | 48 | 222 | 335 | 881 | 455 | 619 |
D | 77 | 253 | 362 | 893 | 467 | 621 |
E | 84 | 256 | 368 | 894 | 503 | 659 |
F | 85 | 258 | 388 | 895 | 524 | 686 |
G | 107 | 280 | 395 | 967 | 538 | 736 |
H | 198 | 282 | 436 | 970 | 569 | 743 |