Rui:SingelCell: Difference between revisions

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==Background==
==Background==
*Goal: the smallest functional unit; the exact quantity; all full-length RNAs; all classes; at single-base resolution
*Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution


*Step 1: single cell; exact quantity; the 3' end; mRNA; RNAseq
*Step 1: single cell; exact quantity; the 3' end; mRNA; RNAseq

Revision as of 20:39, 9 January 2011

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Step 1: single cell; exact quantity; the 3' end; mRNA; RNAseq

Protocols

PCR-based protocol

IVT-based protocol

Circulation-based protocol

2011

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