Rui:LabNotes/SingleCell/2011-1-26: Difference between revisions

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>RuiLiu
Line 20: Line 20:
  Aim 2: check primer hetero-dimer
  Aim 2: check primer hetero-dimer
  SYBR(-) and Taq(-) are negative control
  SYBR(-) and Taq(-) are negative control
[[File:tube1-4]]
[[File:tube5-8]]
[[File:tube9-10]]

Revision as of 22:37, 26 January 2011

Trouble shooting for PCR

Components in tubes
----------------------------------------------------------------------------------
ID	 Template      P1   P2   others   Taq	SYBR
---------------------------------------------------------------------------------- 
Tube1	 Rui water    Yes   Yes 	  Yes   Yes            
Tube2   Kun water    Yes   Yes  	  Yes   Yes     	        
Tube3   Sam water    Yes   Yes           Yes   Yes
Tube4   10pg RNA     Yes   Yes           Yes   Yes
Tube5   Rui water    Yes                 Yes   Yes
Tube6   Rui water          Yes           Yes   Yes
Tube7   Rui water               P3+P4    Yes   Yes
Tube8   Rui water               P3+p5    Yes   Yes
Tube9   Rui water                              Yes
Tube10	 Rui water                        Yes   	                         	         
----------------------------------------------------------------------------------
Common components: Ex Taq buffer, dNTP mix
Aim 1: check contamination in Rui water; 10pg RNA sample
Aim 2: check primer hetero-dimer
SYBR(-) and Taq(-) are negative control


File:Tube1-4 File:Tube5-8 File:Tube9-10