Rui:LabNotes/SingleCell/2011-1-26: Difference between revisions
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[[File:tube1-4]] | [[File:tube1-4.jpg]] | ||
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[[File:tube9-10]] | [[File:tube9-10.jpg]] |
Revision as of 22:39, 26 January 2011
Trouble shooting for PCR
Components in tubes ---------------------------------------------------------------------------------- ID Template P1 P2 others Taq SYBR ---------------------------------------------------------------------------------- Tube1 Rui water Yes Yes Yes Yes Tube2 Kun water Yes Yes Yes Yes Tube3 Sam water Yes Yes Yes Yes Tube4 10pg RNA Yes Yes Yes Yes Tube5 Rui water Yes Yes Yes Tube6 Rui water Yes Yes Yes Tube7 Rui water P3+P4 Yes Yes Tube8 Rui water P3+p5 Yes Yes Tube9 Rui water Yes Tube10 Rui water Yes ---------------------------------------------------------------------------------- Common components: Ex Taq buffer, dNTP mix Aim 1: check contamination in Rui water; 10pg RNA sample Aim 2: check primer hetero-dimer SYBR(-) and Taq(-) are negative control
File:Tube1-4.jpg File:Tube5-8.jpg File:Tube9-10.jpg