Rui:LabNotes/SingleCell/2011-1-27: Difference between revisions

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>RuiLiu
(Created page with '==Titration of Exonuclease I == *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and the following step *…')
 
>RuiLiu
Line 1: Line 1:
==Titration of Exonuclease I ==
==Titration of Exonuclease I ==
  *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and the following step
  *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps
  *Positive control: diluted cDNA w/o primer
  *Positive control: diluted cDNA w/o primer
  *Negative control: Rui water
  *Negative control: Rui water
  *Additional note: ds DNA treatment for single cell???
  *Additional note: ds DNA treatment for single cell???

Revision as of 17:49, 27 January 2011

Titration of Exonuclease I

*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps
*Positive control: diluted cDNA w/o primer
*Negative control: Rui water
*Additional note: ds DNA treatment for single cell???