Rui:LabNotes/SingleCell/2011-1-27: Difference between revisions
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>RuiLiu (Created page with '==Titration of Exonuclease I == *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and the following step *…') |
>RuiLiu |
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==Titration of Exonuclease I == | ==Titration of Exonuclease I == | ||
*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and | *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps | ||
*Positive control: diluted cDNA w/o primer | *Positive control: diluted cDNA w/o primer | ||
*Negative control: Rui water | *Negative control: Rui water | ||
*Additional note: ds DNA treatment for single cell??? | *Additional note: ds DNA treatment for single cell??? |
Revision as of 17:49, 27 January 2011
Titration of Exonuclease I
*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps *Positive control: diluted cDNA w/o primer *Negative control: Rui water *Additional note: ds DNA treatment for single cell???