Rui:LabNotes/SingleCell/2011-1-27: Difference between revisions

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==Characterization of positive control==
*Pure cDNA w/o primer carry-over
*1st round of AMpure beads purification, 20ul --> 36ul beads (1.8*) --> 36ul water
*2nd round of AMpure beads purification, 30ul --> 21ul beads (0.7* suggested by Alice) --> 36ul water
Concentration  Nanodrop [ng/ul]
1st Hues54      38.67 (2.16; 2.07)
    Hues6-ES    58.78
    Hues6-EB
==Titration of Exonuclease I ==
==Titration of Exonuclease I ==
  *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps
  *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps

Revision as of 21:30, 27 January 2011

Characterization of positive control

*Pure cDNA w/o primer carry-over
*1st round of AMpure beads purification, 20ul --> 36ul beads (1.8*) --> 36ul water
*2nd round of AMpure beads purification, 30ul --> 21ul beads (0.7* suggested by Alice) --> 36ul water
Concentration   Nanodrop [ng/ul]
1st Hues54      38.67 (2.16; 2.07)
    Hues6-ES    58.78
    Hues6-EB


Titration of Exonuclease I

*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps
*Positive control: diluted cDNA w/o primer
*Negative control: Rui water
*Additional note: ds DNA treatment for single cell???