Rui:LabNotes/SingleCell/2011-1-27: Difference between revisions
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>RuiLiu |
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==Characterization of positive control== | |||
*Pure cDNA w/o primer carry-over | |||
*1st round of AMpure beads purification, 20ul --> 36ul beads (1.8*) --> 36ul water | |||
*2nd round of AMpure beads purification, 30ul --> 21ul beads (0.7* suggested by Alice) --> 36ul water | |||
Concentration Nanodrop [ng/ul] | |||
1st Hues54 38.67 (2.16; 2.07) | |||
Hues6-ES 58.78 | |||
Hues6-EB | |||
==Titration of Exonuclease I == | ==Titration of Exonuclease I == | ||
*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps | *Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps |
Revision as of 21:30, 27 January 2011
Characterization of positive control
*Pure cDNA w/o primer carry-over *1st round of AMpure beads purification, 20ul --> 36ul beads (1.8*) --> 36ul water *2nd round of AMpure beads purification, 30ul --> 21ul beads (0.7* suggested by Alice) --> 36ul water
Concentration Nanodrop [ng/ul] 1st Hues54 38.67 (2.16; 2.07) Hues6-ES 58.78 Hues6-EB
Titration of Exonuclease I
*Goal: high enough to remove primer/unspecific amplification on NTC; low enough to avoid interference to cDNA stability and following steps *Positive control: diluted cDNA w/o primer *Negative control: Rui water *Additional note: ds DNA treatment for single cell???