Alice:LabNotes/2008-5-8: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Zsakura2
(New page: ===PCR=== reaction system x2 H2O 32.1ul 64.2ul 10x JumpStartT...)
 
>Zsakura2
No edit summary
Line 1: Line 1:
===PCR===
===PCR on 27k probe (15ng)===
    
    
   reaction system                                                x2               
   reaction system                                                x2               

Revision as of 01:48, 9 May 2008

PCR on 27k probe (15ng)

 reaction system                                                 x2              
 H2O                                                32.1ul     64.2ul     
 10x JumpStartTaq Buffer                               5ul      10ul     
 MgCl2(25mM)                                           3ul      6ul        
 dNTP(10mM)                                            2ul      4ul          
 AmpF6.2/R6.2 (10uM)                                   2ul      4ul     
 50x SYBG I                                          0.4ul     0.8ul     
 JumpStartTaq                                        0.5ul      1ul     
 Products of Circluarization                           5ul      10ul      
 Total                                                50ul      100ul

94C 3min -> 20 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 30 sec -> 72 30 sec) -> 72C 3min -> 4C hold