Alice:LabNotes/2008-5-8: Difference between revisions
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>Zsakura2 (New page: ===PCR=== reaction system x2 H2O 32.1ul 64.2ul 10x JumpStartT...) |
>Jie deng No edit summary |
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===PCR=== | ===PCR on 27k probe (15ng)=== | ||
reaction system x2 | reaction system x2 | ||
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94C 3min -> 20 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 30 sec -> 72 30 sec) -> 72C 3min -> 4C hold | 94C 3min -> 20 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 30 sec -> 72 30 sec) -> 72C 3min -> 4C hold | ||
[[Image:ZhangLab_2 2008-05-09 16hr 32min.jpeg]] |
Latest revision as of 01:15, 23 May 2008
PCR on 27k probe (15ng)[edit]
reaction system x2 H2O 32.1ul 64.2ul 10x JumpStartTaq Buffer 5ul 10ul MgCl2(25mM) 3ul 6ul dNTP(10mM) 2ul 4ul AmpF6.2/R6.2 (10uM) 2ul 4ul 50x SYBG I 0.4ul 0.8ul JumpStartTaq 0.5ul 1ul Products of Circluarization 5ul 10ul Total 50ul 100ul
94C 3min -> 20 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 30 sec -> 72 30 sec) -> 72C 3min -> 4C hold