AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions
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*Nextera Sample Prep Kit | *Nextera Sample Prep Kit | ||
*Input DNA | *Input DNA | ||
* | *Bisulfite conversion kit | ||
*Qiagen Purification Kit | |||
==Protocol== | ==Protocol== |
Revision as of 01:19, 15 February 2011
Nextera Methyl-Seq DNA Sample Prep
Objective
- Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP
Samples & Materials
- Nextera Sample Prep Kit
- Input DNA
- Bisulfite conversion kit
- Qiagen Purification Kit
Protocol
- Nuclease Free water
100ng input DNA 4ul 5x Nextera HMW Reaction Buffer 1ul Nextera Methyl-Seq Tagmentation Mix