Athurva Gore/LabNotes/2011-4-8: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Ajgore
>Ajgore
No edit summary
Line 2: Line 2:
* Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:
* Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:
[[File:04072011_Low_Input_Bisulfite_FAILED.jpg|500px]]
[[File:04072011_Low_Input_Bisulfite_FAILED.jpg|500px]]
* Bisulfite-converted libraries did not work.
** DNA was definitely attached to beads, as normal amplification worked.
** DNA must have been detached
* '''Can we modify the experiment to still allow attachment to beads?'''
** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when:
*** fds

Revision as of 21:57, 8 April 2011

Bisulfite Biotin-Binding

  • Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:

File:04072011 Low Input Bisulfite FAILED.jpg

  • Bisulfite-converted libraries did not work.
    • DNA was definitely attached to beads, as normal amplification worked.
    • DNA must have been detached
  • Can we modify the experiment to still allow attachment to beads?
    • According to Invitrogen website [1], bead-detachment can occur when:
      • fds