Athurva Gore/LabNotes/2011-4-8: Difference between revisions
Jump to navigation
Jump to search
>Ajgore |
>Ajgore No edit summary |
||
Line 2: | Line 2: | ||
* Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA: | * Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA: | ||
[[File:04072011_Low_Input_Bisulfite_FAILED.jpg|500px]] | [[File:04072011_Low_Input_Bisulfite_FAILED.jpg|500px]] | ||
* Bisulfite-converted libraries did not work. | |||
** DNA was definitely attached to beads, as normal amplification worked. | |||
** DNA must have been detached | |||
* '''Can we modify the experiment to still allow attachment to beads?''' | |||
** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when: | |||
*** fds |
Revision as of 21:57, 8 April 2011
Bisulfite Biotin-Binding
- Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:
File:04072011 Low Input Bisulfite FAILED.jpg
- Bisulfite-converted libraries did not work.
- DNA was definitely attached to beads, as normal amplification worked.
- DNA must have been detached
- Can we modify the experiment to still allow attachment to beads?
- According to Invitrogen website [1], bead-detachment can occur when:
- fds
- According to Invitrogen website [1], bead-detachment can occur when: