Rui:LabNotes/SingleCell/2011-5-20: Difference between revisions

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==Additional 10 cycles of direct lysis samples==
* Continue with lab note on 5.19.11
* Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA
===QPCR curve===
[[File:additional 10 cycles_5.20.11.jpg]]
===TBE PAGE===
[[File:10 cycles_5.20.11.jpg]]
==Repeat 2nd strands and PCR with the remaining samples==
* Continue with samples on 5.19.11, the remaining 9ul A-tailing product
* 2nd strand with P8
* PCR with 30 cycles

Latest revision as of 18:20, 17 June 2011