Rui:LabNotes/SingleCell/2011-5-21: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>RuiLiu
(Created page with '==Repeat 2nd strands and PCR with the remaining samples== ===QPCR curve=== ===Beads purification=== * Combined into 4 samples, 8 PCR tubes per samples * Sperm RNA, ES RNA, Sper…')
 
>RuiLiu
(Blanked the page)
 
Line 1: Line 1:
==Repeat 2nd strands and PCR with the remaining samples==


===QPCR curve===
===Beads purification===
* Combined into 4 samples, 8 PCR tubes per samples
* Sperm RNA, ES RNA, Sperm lysis, ES lysis
* 0.7X beads purification
* Elute with 50ul water
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''ng/ul'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''260/230'''
|-
| sperm RNA||43.64||1.9||1.68
|-
| ES RNA||295.67||1.93||2.18
|-
| ES lysis||220.08||1.92||1.97
|-
| sperm lysis||23.5||1.68||0.95
|-
|
|}
===Cloning===
* Ligation: ~25-30ng purified PCR + 2.5ng vector in 10ul total ligation, 14C, o/n
* Transformation, using Kan30 plate

Latest revision as of 18:22, 17 June 2011