Rui:LabNotes/SingleCell/2011-6-4: Difference between revisions
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>RuiLiu (Created page with '==Kapa PCR for ER EL== * EL sample is on 5.26.11 * ER sample is on 6.2.11 * 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13 ===QPCR curve=== File:QPCR_6.4.11.jpg ===…') |
>RuiLiu No edit summary |
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[[File:TBE PAGE_6.4.11.jpg]] | [[File:TBE PAGE_6.4.11.jpg]] | ||
==Size selection for sequencing== | |||
# run TBE-PAGE, cut bands around 300bp-700bp | |||
# finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer) | |||
# Gel check for selected library was in lab note 6.6.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-6-6#2nd_round_of_PCR] | |||
# Mixed with haplotyping samples for sequencing |
Revision as of 17:54, 13 June 2011
Kapa PCR for ER EL
- EL sample is on 5.26.11
- ER sample is on 6.2.11
- 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13
QPCR curve
TBE PAGE
Size selection for sequencing
- run TBE-PAGE, cut bands around 300bp-700bp
- finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer)
- Gel check for selected library was in lab note 6.6.11 [1]
- Mixed with haplotyping samples for sequencing