Rui:LabNotes/SingleCell/2011-6-4: Difference between revisions
Jump to navigation
Jump to search
>RuiLiu (Created page with '==Kapa PCR for ER EL== * EL sample is on 5.26.11 * ER sample is on 6.2.11 * 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13 ===QPCR curve=== File:QPCR_6.4.11.jpg ===…') |
>RuiLiu |
||
(One intermediate revision by the same user not shown) | |||
Line 11: | Line 11: | ||
[[File:TBE PAGE_6.4.11.jpg]] | [[File:TBE PAGE_6.4.11.jpg]] | ||
==Size selection for sequencing== | |||
# run TBE-PAGE, cut bands around 300bp-700bp | |||
# finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer) | |||
# Gel check for selected library was in lab note 6.6.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-6-6#2nd_round_of_PCR] | |||
# Mixed with haplotyping samples for sequencing, sample ID: '''RL-Hap_RNAseq-1_13-Jun6''' |
Latest revision as of 18:06, 13 June 2011
Kapa PCR for ER EL[edit]
- EL sample is on 5.26.11
- ER sample is on 6.2.11
- 2ul samples in 40ul total. EL - N2.ID12; ER - N2.ID13
QPCR curve[edit]
TBE PAGE[edit]
Size selection for sequencing[edit]
- run TBE-PAGE, cut bands around 300bp-700bp
- finish gel purification procedure in one day (exact 1hr 37C incubation of gel in TE buffer)
- Gel check for selected library was in lab note 6.6.11 [1]
- Mixed with haplotyping samples for sequencing, sample ID: RL-Hap_RNAseq-1_13-Jun6