AlanFung:LabNotes/CTCF/2011-6-7: Difference between revisions

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>Alan6017518
>Alan6017518
Line 32: Line 32:
  72C 10m
  72C 10m
  15C hold
  15C hold
==Results==
[[File:060611_CHR8_pfuCx.jpg|500px]]

Revision as of 20:35, 7 June 2011

qPCR with CHR8 primer using pfu Cx polymerase on bisulfite converted GM12878 gDNA

Protocol

Component amount per reaction
distilled water 35.6
10x pfuturbo cx reaction buffer 5
dNTPs(10mM each DNTP) 1
DNA template 1
CHR8 F primer 3.3uM 3
CHR8 R primer 3.3um 3
sybr green 50x 0.4
pfuturbo cx hotstart dna polymerase 2.5U/ul 1
total reaction volume 50
    • Perform Manufacturer protocol for PfuTurbo Cx Hotstart DNA Polymerase
95C 2m
95C 30s
52C 30s
72C 2m
Plate Read
Go to step 2 repeat for 29 times
72C 10m
15C hold

Results

File:060611 CHR8 pfuCx.jpg