AlanFung:LabNotes/CTCF/2011-6-7: Difference between revisions
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==Results== | ==Results== | ||
[[File:060611_CHR8_pfuCx.jpg|500px]] | [[File:060611_CHR8_pfuCx.jpg|500px]] | ||
*Amplification failed with Pfu cx polymerase |
Revision as of 20:36, 7 June 2011
qPCR with CHR8 primer using pfu Cx polymerase on bisulfite converted GM12878 gDNA
Protocol
Component | amount per reaction |
distilled water | 35.6 |
10x pfuturbo cx reaction buffer | 5 |
dNTPs(10mM each DNTP) | 1 |
DNA template | 1 |
CHR8 F primer 3.3uM | 3 |
CHR8 R primer 3.3um | 3 |
sybr green 50x | 0.4 |
pfuturbo cx hotstart dna polymerase 2.5U/ul | 1 |
total reaction volume | 50 |
- Perform Manufacturer protocol for PfuTurbo Cx Hotstart DNA Polymerase
95C 2m 95C 30s 52C 30s 72C 2m Plate Read Go to step 2 repeat for 29 times 72C 10m 15C hold
Results
- Amplification failed with Pfu cx polymerase