Rui:Collaborations: Difference between revisions

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# GFP(-): Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul
# GFP(-): Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul
# 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan)
# 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan)
  Negative control (No fluorescence)--- 2 tubes
  Negative control (No fluorescence)--- 2 tubes (>200,000 cells each)
  RFP positive ------ 1 tube
  RFP positive ------ 1 tube (~12,000 cells)
  GFP/RFP double positive ------ 1 tube
  GFP/RFP double positive ------ 1 tube (~55,000 cells)


==Quick note==
==Quick note==

Revision as of 19:58, 8 July 2011

Samples

Dr. Yi Zhang lab

  1. Sperms: ~50cell/tube; 15 tubes
  2. ES cells: ~50cell/tube; 15 tubes
  3. E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
  4. E11.5 bag: ~21550 cells in 1 tube.
  5. E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)

Dr. Xu Yang lab

  1. GFP(+): Pancreatic lineage differentiated from hESC, NGN3+ (~50000 cells, purified with Qiagen RNeasy), 20.8ug/ml, use 1ul
  2. GFP(-): Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul
  3. 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan)
Negative control (No fluorescence)--- 2 tubes (>200,000 cells each)
RFP positive ------ 1 tube (~12,000 cells)
GFP/RFP double positive ------ 1 tube (~55,000 cells)

Quick note

MmeI primer digestion [1]

50 cells RNAseq procedure samples start end note
~25 cells RNA or lysate Sperm vs ES cells 5.19.11 5.20.11
process, clone 5.21.11 5.24.11 5.25.11 seq result
Nextera library 5.23.11 5.23.11 spread out (clone?)
Solexa library 5.23.11 5.23.11 amp. Not good
5.26.11 5.26.11 again. Not good
6.1.11 6.2.11 different buffs
6.2.11 6.3.11 another 2ug repeat
6.4.11 Kapa for NGS

2011

<calendar> name=Rui:LabNotes/Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>