Rui:Collaborations: Difference between revisions

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==Dr. Yi Zhang lab==


===Samples===
# Sperms: ~50cell/tube; 15 tubes
# ES cells: ~50cell/tube; 15 tubes
# E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
# E11.5 bag: ~21550 cells in 1 tube.
# E13.5 bag: ~30078 cells in 1 tube (male PGCs);  ~57059 cells in 1 tube (female PGCs)
===Quick note===
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''50 cells RNAseq'''
| align="center" style="background:#f0f0f0;"|'''procedure'''
| align="center" style="background:#f0f0f0;"|'''samples'''
| align="center" style="background:#f0f0f0;"|'''start'''
| align="center" style="background:#f0f0f0;"|'''end'''
| align="center" style="background:#f0f0f0;"|'''note'''
|-
| ||RNA or lysate||Sperm vs ES cells||5.19.11||5.20.11||
|-
| ||||process, clone||5.21.11||5.24.11||5.25.11 seq result
|-
| ||||Nextera library||5.23.11||5.23.11||spread out (clone?)
|-
| ||||Solexa library||5.23.11||5.23.11||amp. Not good
|-
| ||||||5.26.11||5.26.11||again. Not good
|-
| ||||||6.1.11||6.2.11||different buffs
|-
| ||||||6.2.11||6.3.11||another 2ug repeat
|-
| ||||||6.4.11||||Kapa for NGS
|}
===2011===
<calendar>
name=Rui:LabNotes/SingleCell
format=%name/%year-%month-%day
date=2011/10/1
view=oneyear
</calendar>

Latest revision as of 00:53, 7 December 2011