Kun:LabNotes/ASE: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
|||
Line 12: | Line 12: | ||
**I decided to extend the gap size from 1bp to 10bp, which will allow the exclusion of some non-specific circularization products. | **I decided to extend the gap size from 1bp to 10bp, which will allow the exclusion of some non-specific circularization products. | ||
**The capturing arms are Tm normalized with the exception of some SNPs in regions with extreme GC content. | **The capturing arms are Tm normalized with the exception of some SNPs in regions with extreme GC content. | ||
** | **Amplification adaptors and linker: Version 6, same as CES22k. |
Revision as of 02:13, 18 December 2007
<calendar> name=Kun:LabNotes/ASE format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar>
Design of the CES28K probe set
- This is the probe set following the CES22k set. The major differences are:
- The SNPs were chosen based on RefSeq gene instead of the knowngenes table in the GoldenPath database.
- SNPs with MAF>0.05 are included.
- I decided to extend the gap size from 1bp to 10bp, which will allow the exclusion of some non-specific circularization products.
- The capturing arms are Tm normalized with the exception of some SNPs in regions with extreme GC content.
- Amplification adaptors and linker: Version 6, same as CES22k.