Brandon Sos: Difference between revisions

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>Bsos
>Bsos
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[[Brandon:Protocols/MAXIscript_T7_IVT | Invitrogen MAXIscript T7 In vitro transcription kit]]
[[Brandon:Protocols/MAXIscript_T7_IVT | Invitrogen MAXIscript T7 In vitro transcription kit]]
[[Brandon:Protocols/SMART_MMLV_RT | SMART MMLV RT protocol (for first strand synthesis)]]


[[Brandon:Protocols/taq_amplification2 | standard PCR cycling for for different enzymes etc]]
[[Brandon:Protocols/taq_amplification2 | standard PCR cycling for for different enzymes etc]]

Revision as of 19:51, 18 May 2012

Current Projects

Protocols

polyacrylamide DNA gel

TBU gel

Count cells

Primers prep

Lysis buffers

gel size selection

AMPure XP beads purification only

AMPure XP beads purification 2-step PCR

Cloning and Transformation.

Invitrogen MAXIscript T7 In vitro transcription kit

SMART MMLV RT protocol (for first strand synthesis)

standard PCR cycling for for different enzymes etc

  • notes
    • minielute colums for <10 ul samples, otherwise use qiaquick columns


Scratch for notes etc

Main Calendar

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>