Brandon Sos: Difference between revisions

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>Bsos
>Bsos
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[[Brandon:Protocols/egel_protocol | e gel protocol]]
[[Brandon:Protocols/egel_protocol | e gel protocol]]
[[Brandon:Protocols/qubit_protocol | qubit protocol]]


*notes
*notes

Revision as of 22:21, 2 July 2012

Current Projects

Protocols

polyacrylamide DNA gel

TBU gel

Count cells

Primers prep

Lysis buffers

gel size selection

AMPure XP beads purification only

AMPure XP beads purification 2-step PCR

Cloning and Transformation.

Invitrogen MAXIscript T7 In vitro transcription kit

SMART MMLV RT protocol (for first strand synthesis)

standard PCR cycling for for different enzymes etc

enzyme reaction buffer compositions

e gel protocol

qubit protocol


  • notes
    • minielute colums for <10 ul samples, otherwise use qiaquick columns


Scratch for notes etc

Main Calendar

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>