Brandon Sos: Difference between revisions
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[[Brandon:Protocols/agarose_gel_protocol | agarose gel protocol]] | [[Brandon:Protocols/agarose_gel_protocol | agarose gel protocol]] | ||
[[Brandon:Protocols/tn5_production | tn5 production protocol]] | |||
Revision as of 18:32, 17 June 2014
Current Projects
Protocols
AMPure XP beads purification only (for >= 5 ng)
AMPure XP beads purification 2-step PCR (for <=5 ng)
Invitrogen MAXIscript T7 In vitro transcription kit
SMART MMLV RT protocol (for first strand synthesis)
standard PCR cycling for for different enzymes etc
enzyme reaction buffer compositions
- notes
- minielute colums for <10 ul samples, otherwise use qiaquick columns
- N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor
Main Calendar
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2014/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2013/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>