Brandon Sos: Difference between revisions

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[[Brandon:Protocols/tn5_production | tn5 production protocol]]
[[Brandon:Protocols/tn5_production | tn5 production protocol]]
[[Brandon:Protocols/tn5_production | tn5 production protocol updated (7/30/2014)]]





Revision as of 23:48, 30 July 2014

Current Projects

Protocols

polyacrylamide DNA gel

TBU gel

Count cells

Primers prep

Lysis buffers

gel size selection

AMPure XP beads purification only (for >= 5 ng)

AMPure XP beads purification 2-step PCR (for <=5 ng)

Cloning and Transformation.

Invitrogen MAXIscript T7 In vitro transcription kit

SMART MMLV RT protocol (for first strand synthesis)

standard PCR cycling for for different enzymes etc

enzyme reaction buffer compositions

e gel protocol

qubit protocol

agarose gel protocol

tn5 production protocol

tn5 production protocol updated (7/30/2014)


  • notes
    • minielute colums for <10 ul samples, otherwise use qiaquick columns
    • N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor


Scratch for notes etc

Main Calendar

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2014/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2013/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>