Brandon Sos: Difference between revisions
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[[Brandon:Protocols/tn5_production | tn5 production protocol]] | [[Brandon:Protocols/tn5_production | tn5 production protocol]] | ||
[[Brandon:Protocols/tn5_production_updated_7_30_2014 | tn5 production protocol updated (7/30/2014)]] | [[Brandon:Protocols/tn5_production_updated_7_30_2014 | tn5 production protocol updated R3 (7/30/2014)]] | ||
[[Brandon:Protocols/tn5_production_updated_9_3_2014 | tn5 production protocol updated (9/3/2014)]] | [[Brandon:Protocols/tn5_production_updated_9_3_2014 | tn5 production protocol updated R4 (9/3/2014)]] | ||
Revision as of 00:24, 24 September 2014
Current Projects
Protocols
AMPure XP beads purification only (for >= 5 ng)
AMPure XP beads purification 2-step PCR (for <=5 ng)
Invitrogen MAXIscript T7 In vitro transcription kit
SMART MMLV RT protocol (for first strand synthesis)
standard PCR cycling for for different enzymes etc
enzyme reaction buffer compositions
tn5 production protocol updated R3 (7/30/2014)
tn5 production protocol updated R4 (9/3/2014)
- notes
- minielute colums for <10 ul samples, otherwise use qiaquick columns
- N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor
Main Calendar
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2014/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2013/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>