Noi/NOTES/2011-8-14: Difference between revisions
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>Noi (Created page with '= BSPP DMR330k capture of brain sample from Dr. Ophoff's lab (UCLA) = * August 1, 2011, received 96 samples from Dr. Ophoff's lab * Tina Wang confirmed the concentration of each …') |
>Noi No edit summary |
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* Tina Wang confirmed the concentration of each sample was about 50ng/ul | * Tina Wang confirmed the concentration of each sample was about 50ng/ul | ||
* The concentrations were determined again by Nanadrop, and it turned out that the concentrations were vere varied. The volume of the gDNA needed to be adjust when perform bisulfite conversion | * The concentrations were determined again by Nanadrop, and it turned out that the concentrations were vere varied. The volume of the gDNA needed to be adjust when perform bisulfite conversion | ||
* Note: The ratio of 260/230nm of most of the samples were very low, so I assumed higj salt contamination. | |||
== Nanodrop measurement == | == Nanodrop measurement == | ||
{| {{table}} border = 1 | {| {{table}} border = 1 | ||
Line 46: | Line 47: | ||
| H1||108.95||H2||57.54||H3||83.68||H4||90.89||H5||100.31||H6||76.76||H7||55.88||H8||144.05||H9||51.54||H10||70.48||H11||125.43||H12||74.57 | | H1||108.95||H2||57.54||H3||83.68||H4||90.89||H5||100.31||H6||76.76||H7||55.88||H8||144.05||H9||51.54||H10||70.48||H11||125.43||H12||74.57 | ||
|}<br> | |}<br> | ||
= Bisulfite conversion = | |||
* Used EZ-96 DNA Methylation-Gold™ Kit (deep well format) Cat#D5008 | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume for 1200ng''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''H2O''' | |||
| align="center" style="background:#f0f0f0;"|'''Total amount (ng)''' | |||
| align="center" style="background:#f0f0f0;"|'''CT conversion reagent (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total volume (ul)''' | |||
|- | |||
| A1||127.53||9.41||A1||10.59||1200.00||130.00||150.00 | |||
|- | |||
| B1||214.10||5.60||B1||14.40||1200.00||130.00||150.00 | |||
|- | |||
| C1||98.39||12.20||C1||7.80||1200.00||130.00||150.00 | |||
|- | |||
| D1||102.29||11.73||D1||8.27||1200.00||130.00||150.00 | |||
|- | |||
| E1||90.70||13.23||E1||6.77||1200.00||130.00||150.00 | |||
|- | |||
| F1*||55.57||20.00||F1*||0.00||1111.40||130.00||150.00 | |||
|- | |||
| G1||111.01||10.81||G1||9.19||1200.00||130.00||150.00 | |||
|- | |||
| H1||108.95||11.01||H1||8.99||1200.00||130.00||150.00 | |||
|- | |||
| A2||101.77||11.79||A2||8.21||1200.00||130.00||150.00 | |||
|- | |||
| B2||109.05||11.00||B2||9.00||1200.00||130.00||150.00 | |||
|- | |||
| C2||106.42||11.28||C2||8.72||1200.00||130.00||150.00 | |||
|- | |||
| D2||94.38||12.71||D2||7.29||1200.00||130.00||150.00 | |||
|- | |||
| E2||84.67||14.17||E2||5.83||1200.00||130.00||150.00 | |||
|- | |||
| F2||108.48||11.06||F2||8.94||1200.00||130.00||150.00 | |||
|- | |||
| G2||62.01||19.35||G2||0.65||1200.00||130.00||150.00 | |||
|- | |||
| H2*||57.54||20.00||H2*||0.00||1150.80||130.00||150.00 | |||
|- | |||
| A3||104.16||11.52||A3||8.48||1200.00||130.00||150.00 | |||
|- | |||
| B3||128.33||9.35||B3||10.65||1200.00||130.00||150.00 | |||
|- | |||
| C3||90.52||13.26||C3||6.74||1200.00||130.00||150.00 | |||
|- | |||
| D3||75.38||15.92||D3||4.08||1200.00||130.00||150.00 | |||
|- | |||
| E3||139.81||8.58||E3||11.42||1200.00||130.00||150.00 | |||
|- | |||
| F3||67.75||17.71||F3||2.29||1200.00||130.00||150.00 | |||
|- | |||
| G3||98.83||12.14||G3||7.86||1200.00||130.00||150.00 | |||
|- | |||
| H3||83.68||14.34||H3||5.66||1200.00||130.00||150.00 | |||
|- | |||
| A4||89.42||13.42||A4||6.58||1200.00||130.00||150.00 | |||
|- | |||
| B4||80.22||14.96||B4||5.04||1200.00||130.00||150.00 | |||
|- | |||
| C4||114.26||10.50||C4||9.50||1200.00||130.00||150.00 | |||
|- | |||
| D4||119.02||10.08||D4||9.92||1200.00||130.00||150.00 | |||
|- | |||
| E4||90.16||13.31||E4||6.69||1200.00||130.00||150.00 | |||
|- | |||
| F4||117.73||10.19||F4||9.81||1200.00||130.00||150.00 | |||
|- | |||
| G4||133.36||9.00||G4||11.00||1200.00||130.00||150.00 | |||
|- | |||
| H4||90.89||13.20||H4||6.80||1200.00||130.00||150.00 | |||
|- | |||
| A5||108.53||11.06||A5||8.94||1200.00||130.00||150.00 | |||
|- | |||
| B5||108.85||11.02||B5||8.98||1200.00||130.00||150.00 | |||
|- | |||
| C5||100.28||11.97||C5||8.03||1200.00||130.00||150.00 | |||
|- | |||
| D5||109.51||10.96||D5||9.04||1200.00||130.00||150.00 | |||
|- | |||
| E5||98.92||12.13||E5||7.87||1200.00||130.00||150.00 | |||
|- | |||
| F5||114.30||10.50||F5||9.50||1200.00||130.00||150.00 | |||
|- | |||
| G5||166.66||7.20||G5||12.80||1200.00||130.00||150.00 | |||
|- | |||
| H5||100.31||11.96||H5||8.04||1200.00||130.00||150.00 | |||
|- | |||
| A6||98.50||12.18||A6||7.82||1200.00||130.00||150.00 | |||
|- | |||
| B6||74.95||16.01||B6||3.99||1200.00||130.00||150.00 | |||
|- | |||
| C6||91.90||13.06||C6||6.94||1200.00||130.00||150.00 | |||
|- | |||
| D6||63.31||18.95||D6||1.05||1200.00||130.00||150.00 | |||
|- | |||
| E6||76.35||15.72||E6||4.28||1200.00||130.00||150.00 | |||
|- | |||
| F6||77.34||15.52||F6||4.48||1200.00||130.00||150.00 | |||
|- | |||
| G6||100.09||11.99||G6||8.01||1200.00||130.00||150.00 | |||
|- | |||
| H6||76.76||15.63||H6||4.37||1200.00||130.00||150.00 | |||
|- | |||
| A7||71.86||16.70||A7||3.30||1200.00||130.00||150.00 | |||
|- | |||
| B7**||39.82||20.00||B7**||0.00||796.40||130.00||150.00 | |||
|- | |||
| C7||79.72||15.05||C7||4.95||1200.00||130.00||150.00 | |||
|- | |||
| D7||100.77||11.91||D7||8.09||1200.00||130.00||150.00 | |||
|- | |||
| E7||197.06||6.09||E7||13.91||1200.00||130.00||150.00 | |||
|- | |||
| F7||60.37||19.88||F7||0.12||1200.00||130.00||150.00 | |||
|- | |||
| G7*||53.66||20.00||G7*||0.00||1073.20||130.00||150.00 | |||
|- | |||
| H8*||55.88||20.00||H8*||0.00||1117.60||130.00||150.00 | |||
|- | |||
| A8*||59.88||20.00||A8*||0.00||1197.60||130.00||150.00 | |||
|- | |||
| B8||85.67||14.01||B8||5.99||1200.00||130.00||150.00 | |||
|- | |||
| C8||96.65||12.42||C8||7.58||1200.00||130.00||150.00 | |||
|- | |||
| D8||128.37||9.35||D8||10.65||1200.00||130.00||150.00 | |||
|- | |||
| E8||89.01||13.48||E8||6.52||1200.00||130.00||150.00 | |||
|- | |||
| F8||95.33||12.59||F8||7.41||1200.00||130.00||150.00 | |||
|- | |||
| G8||84.04||14.28||G8||5.72||1200.00||130.00||150.00 | |||
|- | |||
| H8||144.05||8.33||H8||11.67||1200.00||130.00||150.00 | |||
|- | |||
| A9||76.42||15.70||A9||4.30||1200.00||130.00||150.00 | |||
|- | |||
| B9||228.10||5.26||B9||14.74||1200.00||130.00||150.00 | |||
|- | |||
| C9||119.19||10.07||C9||9.93||1200.00||130.00||150.00 | |||
|- | |||
| D9||269.45||4.45||D9||15.55||1200.00||130.00||150.00 | |||
|- | |||
| E9*||49.91||20.00||E9*||0.00||998.20||130.00||150.00 | |||
|- | |||
| F9||86.00||13.95||F9||6.05||1200.00||130.00||150.00 | |||
|- | |||
| G9***||593.63||2.02||G9***||17.98||1200.00||130.00||150.00 | |||
|- | |||
| H9*||51.54||20.00||H9*||0.00||1030.80||130.00||150.00 | |||
|- | |||
| A10||59.93||20.00||A10||0.00||1198.60||130.00||150.00 | |||
|- | |||
| B10||72.25||16.61||B10||3.39||1200.00||130.00||150.00 | |||
|- | |||
| C10||69.85||17.18||C10||2.82||1200.00||130.00||150.00 | |||
|- | |||
| D10||129.33||9.28||D10||10.72||1200.00||130.00||150.00 | |||
|- | |||
| E10||62.05||19.34||E10||0.66||1200.00||130.00||150.00 | |||
|- | |||
| F10||139.09||8.63||F10||11.37||1200.00||130.00||150.00 | |||
|- | |||
| G10||189.64||6.33||G10||13.67||1200.00||130.00||150.00 | |||
|- | |||
| H10||70.48||17.03||H10||2.97||1200.00||130.00||150.00 | |||
|- | |||
| A11||152.47||7.87||A11||12.13||1200.00||130.00||150.00 | |||
|- | |||
| B11||90.51||13.26||B11||6.74||1200.00||130.00||150.00 | |||
|- | |||
| C11||106.38||11.28||C11||8.72||1200.00||130.00||150.00 | |||
|- | |||
| D11||88.70||13.53||D11||6.47||1200.00||130.00||150.00 | |||
|- | |||
| E11||78.12||15.36||E11||4.64||1200.00||130.00||150.00 | |||
|- | |||
| F11||61.46||19.52||F11||0.48||1200.00||130.00||150.00 | |||
|- | |||
| G11||111.77||10.74||G11||9.26||1200.00||130.00||150.00 | |||
|- | |||
| H11||125.43||9.57||H11||10.43||1200.00||130.00||150.00 | |||
|- | |||
| A12||74.20||16.17||A12||3.83||1200.00||130.00||150.00 | |||
|- | |||
| B12||92.20||13.02||B12||6.98||1200.00||130.00||150.00 | |||
|- | |||
| C12**||46.85||20.00||C12*||0.00||937.00||130.00||150.00 | |||
|- | |||
| D12||68.39||17.55||D12||2.45||1200.00||130.00||150.00 | |||
|- | |||
| E12||83.77||14.32||E12||5.68||1200.00||130.00||150.00 | |||
|- | |||
| F12*||53.64||20.00||F12*||0.00||1072.80||130.00||150.00 | |||
|- | |||
| G12||106.40||11.28||G12||8.72||1200.00||130.00||150.00 | |||
|- | |||
| H12||74.57||16.09||H12||3.91||1200.00||130.00||150.00 | |||
|}<br> | |||
* Eluted volume 20ul with Elution buffer. After Qubit ss-DNA assay the total yields will be calculated based on ~16ul left in the well. | |||
* Note: | |||
** *= Total amount in bis-cvt lower than 1200ng, but still above 1000ng | |||
** ** or ***= Performed two bis-cvt (one in the plate and one in the column (B7 (conc, too low, G9 (conc. too high) | |||
** ***= conc. too high |
Revision as of 18:09, 14 August 2011
BSPP DMR330k capture of brain sample from Dr. Ophoff's lab (UCLA)
- August 1, 2011, received 96 samples from Dr. Ophoff's lab
- Tina Wang confirmed the concentration of each sample was about 50ng/ul
- The concentrations were determined again by Nanadrop, and it turned out that the concentrations were vere varied. The volume of the gDNA needed to be adjust when perform bisulfite conversion
- Note: The ratio of 260/230nm of most of the samples were very low, so I assumed higj salt contamination.
Nanodrop measurement
Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul | Well ID | ng/ul |
A1 | 127.53 | A2 | 101.77 | A3 | 104.16 | A4 | 89.42 | A5 | 108.53 | A6 | 98.50 | A7 | 71.86 | A8 | 59.88 | A9 | 76.42 | A10 | 59.93 | A11 | 152.47 | A12 | 74.20 |
B1 | 214.10 | B2 | 109.05 | B3 | 128.33 | B4 | 80.22 | B5 | 108.85 | B6 | 74.95 | B7 | 39.82 | B8 | 85.67 | B9 | 228.10 | B10 | 72.25 | B11 | 90.51 | B12 | 92.20 |
C1 | 98.39 | C2 | 106.42 | C3 | 90.52 | C4 | 114.26 | C5 | 100.28 | C6 | 91.90 | C7 | 79.72 | C8 | 96.65 | C9 | 119.19 | C10 | 69.85 | C11 | 106.38 | C12 | 46.85 |
D1 | 102.29 | D2 | 94.38 | D3 | 75.38 | D4 | 119.02 | D5 | 109.51 | D6 | 63.31 | D7 | 100.77 | D8 | 128.37 | D9 | 269.45 | D10 | 129.33 | D11 | 88.70 | D12 | 68.39 |
E1 | 90.70 | E2 | 84.67 | E3 | 139.81 | E4 | 90.16 | E5 | 98.92 | E6 | 76.35 | E7 | 197.06 | E8 | 89.01 | E9 | 49.91 | E10 | 62.05 | E11 | 78.12 | E12 | 83.77 |
F1 | 55.57 | F2 | 108.48 | F3 | 67.75 | F4 | 117.73 | F5 | 114.30 | F6 | 77.34 | F7 | 60.37 | F8 | 95.33 | F9 | 86.00 | F10 | 139.09 | F11 | 61.46 | F12 | 53.64 |
G1 | 111.01 | G2 | 62.01 | G3 | 98.83 | G4 | 133.36 | G5 | 166.66 | G6 | 100.09 | G7 | 53.66 | G8 | 84.04 | G9 | 593.63 | G10 | 189.64 | G11 | 111.77 | G12 | 106.40 |
H1 | 108.95 | H2 | 57.54 | H3 | 83.68 | H4 | 90.89 | H5 | 100.31 | H6 | 76.76 | H7 | 55.88 | H8 | 144.05 | H9 | 51.54 | H10 | 70.48 | H11 | 125.43 | H12 | 74.57 |
Bisulfite conversion
- Used EZ-96 DNA Methylation-Gold™ Kit (deep well format) Cat#D5008
Sample ID | ng/ul | Volume for 1200ng | Sample ID | H2O | Total amount (ng) | CT conversion reagent (ul) | Total volume (ul) |
A1 | 127.53 | 9.41 | A1 | 10.59 | 1200.00 | 130.00 | 150.00 |
B1 | 214.10 | 5.60 | B1 | 14.40 | 1200.00 | 130.00 | 150.00 |
C1 | 98.39 | 12.20 | C1 | 7.80 | 1200.00 | 130.00 | 150.00 |
D1 | 102.29 | 11.73 | D1 | 8.27 | 1200.00 | 130.00 | 150.00 |
E1 | 90.70 | 13.23 | E1 | 6.77 | 1200.00 | 130.00 | 150.00 |
F1* | 55.57 | 20.00 | F1* | 0.00 | 1111.40 | 130.00 | 150.00 |
G1 | 111.01 | 10.81 | G1 | 9.19 | 1200.00 | 130.00 | 150.00 |
H1 | 108.95 | 11.01 | H1 | 8.99 | 1200.00 | 130.00 | 150.00 |
A2 | 101.77 | 11.79 | A2 | 8.21 | 1200.00 | 130.00 | 150.00 |
B2 | 109.05 | 11.00 | B2 | 9.00 | 1200.00 | 130.00 | 150.00 |
C2 | 106.42 | 11.28 | C2 | 8.72 | 1200.00 | 130.00 | 150.00 |
D2 | 94.38 | 12.71 | D2 | 7.29 | 1200.00 | 130.00 | 150.00 |
E2 | 84.67 | 14.17 | E2 | 5.83 | 1200.00 | 130.00 | 150.00 |
F2 | 108.48 | 11.06 | F2 | 8.94 | 1200.00 | 130.00 | 150.00 |
G2 | 62.01 | 19.35 | G2 | 0.65 | 1200.00 | 130.00 | 150.00 |
H2* | 57.54 | 20.00 | H2* | 0.00 | 1150.80 | 130.00 | 150.00 |
A3 | 104.16 | 11.52 | A3 | 8.48 | 1200.00 | 130.00 | 150.00 |
B3 | 128.33 | 9.35 | B3 | 10.65 | 1200.00 | 130.00 | 150.00 |
C3 | 90.52 | 13.26 | C3 | 6.74 | 1200.00 | 130.00 | 150.00 |
D3 | 75.38 | 15.92 | D3 | 4.08 | 1200.00 | 130.00 | 150.00 |
E3 | 139.81 | 8.58 | E3 | 11.42 | 1200.00 | 130.00 | 150.00 |
F3 | 67.75 | 17.71 | F3 | 2.29 | 1200.00 | 130.00 | 150.00 |
G3 | 98.83 | 12.14 | G3 | 7.86 | 1200.00 | 130.00 | 150.00 |
H3 | 83.68 | 14.34 | H3 | 5.66 | 1200.00 | 130.00 | 150.00 |
A4 | 89.42 | 13.42 | A4 | 6.58 | 1200.00 | 130.00 | 150.00 |
B4 | 80.22 | 14.96 | B4 | 5.04 | 1200.00 | 130.00 | 150.00 |
C4 | 114.26 | 10.50 | C4 | 9.50 | 1200.00 | 130.00 | 150.00 |
D4 | 119.02 | 10.08 | D4 | 9.92 | 1200.00 | 130.00 | 150.00 |
E4 | 90.16 | 13.31 | E4 | 6.69 | 1200.00 | 130.00 | 150.00 |
F4 | 117.73 | 10.19 | F4 | 9.81 | 1200.00 | 130.00 | 150.00 |
G4 | 133.36 | 9.00 | G4 | 11.00 | 1200.00 | 130.00 | 150.00 |
H4 | 90.89 | 13.20 | H4 | 6.80 | 1200.00 | 130.00 | 150.00 |
A5 | 108.53 | 11.06 | A5 | 8.94 | 1200.00 | 130.00 | 150.00 |
B5 | 108.85 | 11.02 | B5 | 8.98 | 1200.00 | 130.00 | 150.00 |
C5 | 100.28 | 11.97 | C5 | 8.03 | 1200.00 | 130.00 | 150.00 |
D5 | 109.51 | 10.96 | D5 | 9.04 | 1200.00 | 130.00 | 150.00 |
E5 | 98.92 | 12.13 | E5 | 7.87 | 1200.00 | 130.00 | 150.00 |
F5 | 114.30 | 10.50 | F5 | 9.50 | 1200.00 | 130.00 | 150.00 |
G5 | 166.66 | 7.20 | G5 | 12.80 | 1200.00 | 130.00 | 150.00 |
H5 | 100.31 | 11.96 | H5 | 8.04 | 1200.00 | 130.00 | 150.00 |
A6 | 98.50 | 12.18 | A6 | 7.82 | 1200.00 | 130.00 | 150.00 |
B6 | 74.95 | 16.01 | B6 | 3.99 | 1200.00 | 130.00 | 150.00 |
C6 | 91.90 | 13.06 | C6 | 6.94 | 1200.00 | 130.00 | 150.00 |
D6 | 63.31 | 18.95 | D6 | 1.05 | 1200.00 | 130.00 | 150.00 |
E6 | 76.35 | 15.72 | E6 | 4.28 | 1200.00 | 130.00 | 150.00 |
F6 | 77.34 | 15.52 | F6 | 4.48 | 1200.00 | 130.00 | 150.00 |
G6 | 100.09 | 11.99 | G6 | 8.01 | 1200.00 | 130.00 | 150.00 |
H6 | 76.76 | 15.63 | H6 | 4.37 | 1200.00 | 130.00 | 150.00 |
A7 | 71.86 | 16.70 | A7 | 3.30 | 1200.00 | 130.00 | 150.00 |
B7** | 39.82 | 20.00 | B7** | 0.00 | 796.40 | 130.00 | 150.00 |
C7 | 79.72 | 15.05 | C7 | 4.95 | 1200.00 | 130.00 | 150.00 |
D7 | 100.77 | 11.91 | D7 | 8.09 | 1200.00 | 130.00 | 150.00 |
E7 | 197.06 | 6.09 | E7 | 13.91 | 1200.00 | 130.00 | 150.00 |
F7 | 60.37 | 19.88 | F7 | 0.12 | 1200.00 | 130.00 | 150.00 |
G7* | 53.66 | 20.00 | G7* | 0.00 | 1073.20 | 130.00 | 150.00 |
H8* | 55.88 | 20.00 | H8* | 0.00 | 1117.60 | 130.00 | 150.00 |
A8* | 59.88 | 20.00 | A8* | 0.00 | 1197.60 | 130.00 | 150.00 |
B8 | 85.67 | 14.01 | B8 | 5.99 | 1200.00 | 130.00 | 150.00 |
C8 | 96.65 | 12.42 | C8 | 7.58 | 1200.00 | 130.00 | 150.00 |
D8 | 128.37 | 9.35 | D8 | 10.65 | 1200.00 | 130.00 | 150.00 |
E8 | 89.01 | 13.48 | E8 | 6.52 | 1200.00 | 130.00 | 150.00 |
F8 | 95.33 | 12.59 | F8 | 7.41 | 1200.00 | 130.00 | 150.00 |
G8 | 84.04 | 14.28 | G8 | 5.72 | 1200.00 | 130.00 | 150.00 |
H8 | 144.05 | 8.33 | H8 | 11.67 | 1200.00 | 130.00 | 150.00 |
A9 | 76.42 | 15.70 | A9 | 4.30 | 1200.00 | 130.00 | 150.00 |
B9 | 228.10 | 5.26 | B9 | 14.74 | 1200.00 | 130.00 | 150.00 |
C9 | 119.19 | 10.07 | C9 | 9.93 | 1200.00 | 130.00 | 150.00 |
D9 | 269.45 | 4.45 | D9 | 15.55 | 1200.00 | 130.00 | 150.00 |
E9* | 49.91 | 20.00 | E9* | 0.00 | 998.20 | 130.00 | 150.00 |
F9 | 86.00 | 13.95 | F9 | 6.05 | 1200.00 | 130.00 | 150.00 |
G9*** | 593.63 | 2.02 | G9*** | 17.98 | 1200.00 | 130.00 | 150.00 |
H9* | 51.54 | 20.00 | H9* | 0.00 | 1030.80 | 130.00 | 150.00 |
A10 | 59.93 | 20.00 | A10 | 0.00 | 1198.60 | 130.00 | 150.00 |
B10 | 72.25 | 16.61 | B10 | 3.39 | 1200.00 | 130.00 | 150.00 |
C10 | 69.85 | 17.18 | C10 | 2.82 | 1200.00 | 130.00 | 150.00 |
D10 | 129.33 | 9.28 | D10 | 10.72 | 1200.00 | 130.00 | 150.00 |
E10 | 62.05 | 19.34 | E10 | 0.66 | 1200.00 | 130.00 | 150.00 |
F10 | 139.09 | 8.63 | F10 | 11.37 | 1200.00 | 130.00 | 150.00 |
G10 | 189.64 | 6.33 | G10 | 13.67 | 1200.00 | 130.00 | 150.00 |
H10 | 70.48 | 17.03 | H10 | 2.97 | 1200.00 | 130.00 | 150.00 |
A11 | 152.47 | 7.87 | A11 | 12.13 | 1200.00 | 130.00 | 150.00 |
B11 | 90.51 | 13.26 | B11 | 6.74 | 1200.00 | 130.00 | 150.00 |
C11 | 106.38 | 11.28 | C11 | 8.72 | 1200.00 | 130.00 | 150.00 |
D11 | 88.70 | 13.53 | D11 | 6.47 | 1200.00 | 130.00 | 150.00 |
E11 | 78.12 | 15.36 | E11 | 4.64 | 1200.00 | 130.00 | 150.00 |
F11 | 61.46 | 19.52 | F11 | 0.48 | 1200.00 | 130.00 | 150.00 |
G11 | 111.77 | 10.74 | G11 | 9.26 | 1200.00 | 130.00 | 150.00 |
H11 | 125.43 | 9.57 | H11 | 10.43 | 1200.00 | 130.00 | 150.00 |
A12 | 74.20 | 16.17 | A12 | 3.83 | 1200.00 | 130.00 | 150.00 |
B12 | 92.20 | 13.02 | B12 | 6.98 | 1200.00 | 130.00 | 150.00 |
C12** | 46.85 | 20.00 | C12* | 0.00 | 937.00 | 130.00 | 150.00 |
D12 | 68.39 | 17.55 | D12 | 2.45 | 1200.00 | 130.00 | 150.00 |
E12 | 83.77 | 14.32 | E12 | 5.68 | 1200.00 | 130.00 | 150.00 |
F12* | 53.64 | 20.00 | F12* | 0.00 | 1072.80 | 130.00 | 150.00 |
G12 | 106.40 | 11.28 | G12 | 8.72 | 1200.00 | 130.00 | 150.00 |
H12 | 74.57 | 16.09 | H12 | 3.91 | 1200.00 | 130.00 | 150.00 |
- Eluted volume 20ul with Elution buffer. After Qubit ss-DNA assay the total yields will be calculated based on ~16ul left in the well.
- Note:
- *= Total amount in bis-cvt lower than 1200ng, but still above 1000ng
- ** or ***= Performed two bis-cvt (one in the plate and one in the column (B7 (conc, too low, G9 (conc. too high)
- ***= conc. too high