Noi/NOTES/2011-9-16: Difference between revisions

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(Created page with '= Plan for BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMALPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymor…')
 
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= Plan for BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMALPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma =
= Plan for BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMALPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma =
* Total number of samples
* Total number of reactions = 39 reactions
** 9 samples from Sergio
** 9 samples from Sergio
** 12 samples from 2 largest pedigrees of HAPMAPPT01  
** 12 samples from 2 largest pedigrees of HAPMAPPT01  
Line 17: Line 17:
**** GM12814 (wellID A7)
**** GM12814 (wellID A7)
**** GM12801 (wellID D9)
**** GM12801 (wellID D9)
** 10 replicates
** 10 replicates
*** NA12156 (HAPMAPPT01)
*** NA12156 (HAPMAPPT01)
** 2 somatic lines  
** 2 somatic lines  
Line 23: Line 23:
*** 218 HFF P7
*** 218 HFF P7
** 4 samples of Penn (repeat the low QC value samples from the first batch)
** 4 samples of Penn (repeat the low QC value samples from the first batch)
** 2 reactions to compare the DNA methylation kit from Zymoresearch (DNA methylation gold kit) and Sigma (Imprint DNA modification kit)
* For the gDNA of HAPMAPPT01, aliquot 10 ug to two working plates and save the original plate

Revision as of 00:47, 16 September 2011

Plan for BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMALPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma

  • Total number of reactions = 39 reactions
    • 9 samples from Sergio
    • 12 samples from 2 largest pedigrees of HAPMAPPT01
      • Family 1362
        • GM11995 (wellID G7)
        • GM11993 (wellID F2)
        • GM10861 (wellID A9)
        • GM11992 (wellID C2 )
        • GM11994 (wellID G9 )
        • GM10860 (wellID F10)
      • Family 1454
        • GM12813 (wellID E8)
        • GM12802 (wellID E7)
        • GM12815 (wellID G12)
        • GM12812 (wellID F11)
        • GM12814 (wellID A7)
        • GM12801 (wellID D9)
    • 10 replicates
      • NA12156 (HAPMAPPT01)
    • 2 somatic lines
      • 2HFF P6
      • 218 HFF P7
    • 4 samples of Penn (repeat the low QC value samples from the first batch)
    • 2 reactions to compare the DNA methylation kit from Zymoresearch (DNA methylation gold kit) and Sigma (Imprint DNA modification kit)
  • For the gDNA of HAPMAPPT01, aliquot 10 ug to two working plates and save the original plate