Noi/NOTES/2011-9-16: Difference between revisions

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= Plan for BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMAPPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma =
= BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMAPPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma =
* ''Total number of reactions = 39 reactions'''
* ''Total number of reactions = 39 reactions'''
** '''9 samples from Sergio'''
** '''9 samples from Sergio'''

Revision as of 01:05, 16 September 2011

BSPP DMR330k capture of samples from Sergio, two largest pedigrees from HAPMAPPT01, ten replicates, two HFF somatic lines, and comparing DNA methylation kit from Zymoresearch and Sigma

  • Total number of reactions = 39 reactions'
    • 9 samples from Sergio
    • 12 samples from 2 largest pedigrees of HAPMAPPT01
      • Family 1362
        • GM11995 (wellID G7)
        • GM11993 (wellID F2)
        • GM10861 (wellID A9)
        • GM11992 (wellID C2 )
        • GM11994 (wellID G9 )
        • GM10860 (wellID F10)
      • Family 1454
        • GM12813 (wellID E8)
        • GM12802 (wellID E7)
        • GM12815 (wellID G12)
        • GM12812 (wellID F11)
        • GM12814 (wellID A7)
        • GM12801 (wellID D9)
    • 10 replicates
      • NA12156 (HAPMAPPT01)
    • 2 somatic lines
      • 2HFF P6
      • 218 HFF P7
    • 4 samples from Penn (repeat the low QC value samples from the first batch)
    • 2 reactions to compare bis-cvt gDNA quality processed by two different DNA methylation kits, Zymoresearch (DNA methylation gold kit) and Sigma (Imprint DNA modification kit)
      • PGP1iPS
  • Note For the gDNA of HAPMAPPT01, aliquot 10 ug to each of two working plates and save the original plate