Rui:LabNotes/Haplotyping/2011-11-3: Difference between revisions

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>RuiLiu
m (Created page with '==Test condition for haplotyping== * Start with ~1pg cell lysate aliquot * Perform limited time MDA * Tagmentation and PCR * Need to achieve ~1000X increase. Based on 50cell expe…')
 
>RuiLiu
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Ctrls [[File:11.3.11_Ctrls.jpg|500px]]  
Ctrls [[File:11.3.11_Ctrls.jpg|500px]]  


stop at 17ct, #2 up [[File:11.3.11_17ct.jpg|500px]] stop at 22ct, #3 up [[File:11.3.11_22ct.jpg| 500px]]  
@17ct, #2 up [[File:11.3.11_17ct.jpg|400px]] @22ct, #3 up [[File:11.3.11_22ct.jpg| 400px]]  


stop at 27ct, #3,4 start to up [[File:11.3.11_27ct.jpg|500px]] stop at 30ct, all start up [[File:11.3.11_30ct.jpg|500px]]
@27ct, #3,4 start to up [[File:11.3.11_27ct.jpg|400px]] @30ct, all start up [[File:11.3.11_30ct.jpg|400px]]

Revision as of 01:21, 4 November 2011

Test condition for haplotyping

  • Start with ~1pg cell lysate aliquot
  • Perform limited time MDA
  • Tagmentation and PCR
  • Need to achieve ~1000X increase. Based on 50cell experiments, it seems 1k-2k increase after 2hrs when QPCR shoots up around 12ct and reach the saturation at 20ct.
  • Key point is when to stop MDA based on QPCR curve

Samples

Procedures

Results

Ctrls File:11.3.11 Ctrls.jpg

@17ct, #2 up File:11.3.11 17ct.jpg @22ct, #3 up File:11.3.11 22ct.jpg

@27ct, #3,4 start to up File:11.3.11 27ct.jpg @30ct, all start up File:11.3.11 30ct.jpg