Rui:LabNotes/Haplotyping/2011-11-3: Difference between revisions
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@27ct, #3,4 start to up [[File:11.3.11_27ct.jpg|400px]] @30ct, all start up [[File:11.3.11_30ct.jpg|400px]] | @27ct, #3,4 start to up [[File:11.3.11_27ct.jpg|400px]] @30ct, all start up [[File:11.3.11_30ct.jpg|400px]] | ||
===EtOH precipitation=== | |||
* Samples: 17-2, 22-3, 27-3,4, 30-1,2,3,4 |
Revision as of 01:22, 4 November 2011
Test condition for haplotyping
- Start with ~1pg cell lysate aliquot
- Perform limited time MDA
- Tagmentation and PCR
- Need to achieve ~1000X increase. Based on 50cell experiments, it seems 1k-2k increase after 2hrs when QPCR shoots up around 12ct and reach the saturation at 20ct.
- Key point is when to stop MDA based on QPCR curve
Samples
Procedures
Results
Ctrls File:11.3.11 Ctrls.jpg
@17ct, #2 up File:11.3.11 17ct.jpg @22ct, #3 up File:11.3.11 22ct.jpg
@27ct, #3,4 start to up File:11.3.11 27ct.jpg @30ct, all start up File:11.3.11 30ct.jpg
EtOH precipitation
- Samples: 17-2, 22-3, 27-3,4, 30-1,2,3,4