Rui:Cell aliquot: Difference between revisions
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>Sam Chiang m (Created page with '===Cell aliquot=== * 11.3.11 GM112878-P28 (~600k cells/ml) GM20431-P20 (~550k cells/ml) # 1ml cells, 750g, 3min # wash pellet with PBS # resuspend in 1ml PBS # 10ul cells + 9…') |
>RuiLiu m (→Cell aliquot) |
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===Cell aliquot=== | ===Cell aliquot=== | ||
* 9.15.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-9-15#Cell_samples] | |||
Alan gave me 2 cell patches: w/o or w/ colcemid treatment | |||
GM12878 P9 - 556,449 cells/ml; GM12878 p9 - 865,509 cells/ml | |||
Collect, wash, resuspend and dilute to 10cell/ul | |||
Aliquot 1ul/tube and store at -80C | |||
* 11.3.11 | * 11.3.11 | ||
GM112878-P28 (~600k cells/ml) | GM112878-P28 (~600k cells/ml) |
Revision as of 01:18, 15 November 2011
Cell aliquot
- 9.15.11 [1]
Alan gave me 2 cell patches: w/o or w/ colcemid treatment GM12878 P9 - 556,449 cells/ml; GM12878 p9 - 865,509 cells/ml Collect, wash, resuspend and dilute to 10cell/ul Aliquot 1ul/tube and store at -80C
- 11.3.11
GM112878-P28 (~600k cells/ml)
GM20431-P20 (~550k cells/ml)
- 1ml cells, 750g, 3min
- wash pellet with PBS
- resuspend in 1ml PBS
- 10ul cells + 90ul PBS -> ~50-60 cells/ul
- 200c strips (2) 4ul/well
- 100c strips (2) 2ul/well
- 50c strips (2) 1ul/well
- 10ul cells + 40ul PBS -> ~10 cells/ul
- 10c strips (4) 1ul/well
- all strips put in tip-box at -80C