Rui:Cell aliquot: Difference between revisions

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>RuiLiu
>RuiLiu
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===Cell aliquot===
===Cell aliquot===
* 9.15.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-9-15#Cell_samples]
* 9.15.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-9-15#Cell_samples]
  Alan gave me 2 cell patches: w/o or w/ colcemid treatment
  Alan gave me 2 cell patches: w/o or w/ colcemid treatment
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# 10ul cells + 90ul PBS -> ~50-60 cells/ul
# 10ul cells + 90ul PBS -> ~50-60 cells/ul
# 200c strips (2) 4ul/well
# 200c strips (2) 4ul/well
# 100c strips (2) 2ul/well
# 50c strips (2) 1ul/well
# 10ul cells + 40ul PBS -> ~10 cells/ul
# 10c strips (4) 1ul/well
* all strips put in tip-box at -80C
* 3.13.12
MEF
# 1ml cells, 750g, 3min
# wash pellet with PBS
# re-suspend in 1ml PBS
# 1000c strips (2) ul/well
# 10ul cells + ul PBS -> ~50 cells/ul
# 500c strips (2) 10ul/well
# 100c strips (2) 2ul/well
# 100c strips (2) 2ul/well
# 50c strips (2) 1ul/well
# 50c strips (2) 1ul/well

Revision as of 23:37, 13 March 2012

Cell aliquot

Alan gave me 2 cell patches: w/o or w/ colcemid treatment
GM12878 P9 - 556,449 cells/ml; GM12878 p9 - 865,509 cells/ml
Collect, wash, resuspend and dilute to 10cell/ul
Aliquot 1ul/tube and store at -80C 
  • 11.3.11

GM112878-P28 (~600k cells/ml)

GM20431-P20 (~550k cells/ml)

  1. 1ml cells, 750g, 3min
  2. wash pellet with PBS
  3. resuspend in 1ml PBS
  4. 10ul cells + 90ul PBS -> ~50-60 cells/ul
  5. 200c strips (2) 4ul/well
  6. 100c strips (2) 2ul/well
  7. 50c strips (2) 1ul/well
  8. 10ul cells + 40ul PBS -> ~10 cells/ul
  9. 10c strips (4) 1ul/well
  • all strips put in tip-box at -80C
  • 3.13.12

MEF

  1. 1ml cells, 750g, 3min
  2. wash pellet with PBS
  3. re-suspend in 1ml PBS
  4. 1000c strips (2) ul/well
  5. 10ul cells + ul PBS -> ~50 cells/ul
  6. 500c strips (2) 10ul/well
  7. 100c strips (2) 2ul/well
  8. 50c strips (2) 1ul/well
  9. 10ul cells + 40ul PBS -> ~10 cells/ul
  10. 10c strips (4) 1ul/well
  • all strips put in tip-box at -80C