Rui:Cell aliquot: Difference between revisions
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>RuiLiu m (→Cell aliquot) |
>RuiLiu m (→Cell aliquot) |
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===Cell aliquot=== | ===Cell aliquot=== | ||
* 9.15.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-9-15#Cell_samples] | * 9.15.11 [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Haplotyping/2011-9-15#Cell_samples] | ||
Alan gave me 2 cell patches: w/o or w/ colcemid treatment | Alan gave me 2 cell patches: w/o or w/ colcemid treatment | ||
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# 10ul cells + 90ul PBS -> ~50-60 cells/ul | # 10ul cells + 90ul PBS -> ~50-60 cells/ul | ||
# 200c strips (2) 4ul/well | # 200c strips (2) 4ul/well | ||
# 100c strips (2) 2ul/well | |||
# 50c strips (2) 1ul/well | |||
# 10ul cells + 40ul PBS -> ~10 cells/ul | |||
# 10c strips (4) 1ul/well | |||
* all strips put in tip-box at -80C | |||
* 3.13.12 | |||
MEF | |||
# 1ml cells, 750g, 3min | |||
# wash pellet with PBS | |||
# re-suspend in 1ml PBS | |||
# 1000c strips (2) ul/well | |||
# 10ul cells + ul PBS -> ~50 cells/ul | |||
# 500c strips (2) 10ul/well | |||
# 100c strips (2) 2ul/well | # 100c strips (2) 2ul/well | ||
# 50c strips (2) 1ul/well | # 50c strips (2) 1ul/well |
Revision as of 23:37, 13 March 2012
Cell aliquot
- 9.15.11 [1]
Alan gave me 2 cell patches: w/o or w/ colcemid treatment GM12878 P9 - 556,449 cells/ml; GM12878 p9 - 865,509 cells/ml Collect, wash, resuspend and dilute to 10cell/ul Aliquot 1ul/tube and store at -80C
- 11.3.11
GM112878-P28 (~600k cells/ml)
GM20431-P20 (~550k cells/ml)
- 1ml cells, 750g, 3min
- wash pellet with PBS
- resuspend in 1ml PBS
- 10ul cells + 90ul PBS -> ~50-60 cells/ul
- 200c strips (2) 4ul/well
- 100c strips (2) 2ul/well
- 50c strips (2) 1ul/well
- 10ul cells + 40ul PBS -> ~10 cells/ul
- 10c strips (4) 1ul/well
- all strips put in tip-box at -80C
- 3.13.12
MEF
- 1ml cells, 750g, 3min
- wash pellet with PBS
- re-suspend in 1ml PBS
- 1000c strips (2) ul/well
- 10ul cells + ul PBS -> ~50 cells/ul
- 500c strips (2) 10ul/well
- 100c strips (2) 2ul/well
- 50c strips (2) 1ul/well
- 10ul cells + 40ul PBS -> ~10 cells/ul
- 10c strips (4) 1ul/well
- all strips put in tip-box at -80C