Rui:LabNotes/Haplotyping/2011-11-4: Difference between revisions
Jump to navigation
Jump to search
>RuiLiu m (Created page with '==Test condition for haplotyping== * Continue with lab note 11.3.11 * Tagmentation and PCR ===Samples=== * Non-MDA ~1pg cell lysates: from same patch of cells as MDA samples, Pt…') |
>Sam Chiang m (→Results) |
||
Line 13: | Line 13: | ||
===Results=== | ===Results=== | ||
MDA samples [[File:11.4.11_MDA-nextera.jpg|400px]] non-MDA samples [[File:11.4.11_ctrls.jpg|400px]] | MDA samples [[File:11.4.11_MDA-nextera.jpg|400px]] non-MDA samples [[File:11.4.11_ctrls.jpg|400px]] | ||
gel check: MDA samples are over-amplified except 2 from 27ct[[File:11.4.11_gel.jpg|300px]] |
Revision as of 23:23, 4 November 2011
Test condition for haplotyping
- Continue with lab note 11.3.11
- Tagmentation and PCR
Samples
- Non-MDA ~1pg cell lysates: from same patch of cells as MDA samples, Ptnase treatment, Nextera
- MDA samples: 1ul each of all 8 MDA product [17ct-2, 22ct-3, 27ct-3,4, 30ct-1,2,3,4] ranging from 1ng - 20ng
Procedure
- Tagmentation: 1ul 1:50d Trp-I, 1ul LMW buffer, fill up to 5ul
- PCR: 15ul Kapa QPCR MM, 0.3ul each B/O primer, fill up to 30ul
Results
MDA samples File:11.4.11 MDA-nextera.jpg non-MDA samples File:11.4.11 ctrls.jpg
gel check: MDA samples are over-amplified except 2 from 27ctFile:11.4.11 gel.jpg