Noi/NOTES/2011-11-15: Difference between revisions

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*Note: The rest of libraries were normalized and pooled for sequencing (some of them were repeated on 2011/10/17)
*Note: The rest of libraries were normalized and pooled for sequencing (some of them were repeated on 2011/10/17)
* Libraries from two families from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma)
* Libraries from two families from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma)
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''# of reads'''
| align="center" style="background:#f0f0f0;"|'''Indx'''
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''Well ID'''
| align="center" style="background:#f0f0f0;"|'''Relative ratio to 4 million reads'''
| align="center" style="background:#f0f0f0;"|'''original volume for 60ng'''
| align="center" style="background:#f0f0f0;"|'''calculated new volume'''
| align="center" style="background:#f0f0f0;"|'''Well ID'''
| align="center" style="background:#f0f0f0;"|'''Well ID'''
|-
| 3,457,940||s_7_1_Indx10||GM11995 (wellID G7)||B3||0.864||10.80||12.49||B3||B3
|-
| 4,062,805||s_7_1_Indx11||GM11993 (wellID F2)||C3||1.016||11.09||10.92||C3||C3
|-
| 3,733,084||s_7_1_Indx12||**GM10861 (wellID A9)||D3||0.933||11.46||12.27||D3||D3
|-
| 3,663,235||s_7_1_Indx13||GM11992 (wellID C2 )||E3||0.916||10.32||11.27||E3||E3
|-
| 2,928,739||s_7_1_Indx14||GM11994 (wellID G9 )||F3||0.732||22.00||30.05||F3||F3
|-
| 7,213,122||s_7_1_Indx15||GM10860 (wellID F10)||G3||1.803||10.99||6.09||G3||G3
|-
| 2,853,891||s_7_1_Indx16||GM12813 (wellID E8)||H3||0.713||6.98||9.79||H3||H3
|-
| 3,678,983||s_7_1_Indx17||GM12802 (wellID E7)||A5||0.920||7.88||8.56||A5||A5
|-
| 3,067,241||s_7_1_Indx18||GM12815 (wellID G12)||B5||0.767||7.48||9.75||B5||B5
|-
| 3,763,585||s_7_1_Indx19||GM12812 (wellID F11)||C5||0.941||10.20||10.84||C5||C5
|-
| 3,246,362||s_7_1_Indx20||GM12814 (wellID A7)||D5||0.812||6.53||8.05||D5||D5
|-
| 3,833,426||s_7_1_Indx21||GM12801 (wellID D9)||E5||0.958||7.08||7.39||E5||E5
|-
| 3,177,943||s_7_1_Indx22||NA12156 (wellID B3)-1||F5||0.794||7.43||9.35||F5||F5
|-
| 3,349,096||s_7_1_Indx23||NA12156 (wellID B3)-2||G5||0.837||6.45||7.70||G5||G5
|-
| 3,604,801||s_7_1_Indx24||NA12156 (wellID B3)-3||H5||0.901||6.49||7.21||H5||H5
|-
| 4,379,684||s_7_1_Indx25||NA12156 (wellID B3)-4||A7||1.095||8.51||7.77||A7||A7
|-
| 3,557,582||s_7_1_Indx26||NA12156 (wellID B3)-5||B7||0.889||10.12||11.38||B7||B7
|-
| 4,331,433||s_7_1_Indx27||NA12156 (wellID B3)-6||C7||1.083||7.49||6.92||C7||C7
|-
| 2,945,843||s_7_1_Indx28||NA12156 (wellID B3)-7||D7||0.736||7.62||10.34||D7||D7
|-
| 3,444,795||s_7_1_Indx29||NA12156 (wellID B3)-8||E7||0.861||9.90||11.50||E7||E7
|-
| 3,429,469||s_7_1_Indx30||NA12156 (wellID B3)-9||F7||0.857||7.39||8.62||F7||F7
|-
| 6,808,403||s_7_1_Indx41||**PGP1iPS||A11||1.702||14.16||8.32||A11||A11
|-
| 5,281,331||s_7_1_Indx42||PGP1iPS-IMPRINT||B11||1.320||17.42||13.19||B11||B11
|}<br>

Revision as of 17:52, 15 November 2011

Normalization of libraries from HL104 run (Library ID: NP-BSPP-Ind1_45-Sep18) to repeat sequencing by GAIIx flowcell

  • Continued from library preparation on 2011/09/18: [[1]] and library normalization on 2011/11/17: [[2]]
  • Note: The rest of libraries were normalized and pooled for sequencing (some of them were repeated on 2011/10/17)
  • Libraries from two families from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma)
# of reads Indx Sample ID Well ID Relative ratio to 4 million reads original volume for 60ng calculated new volume Well ID Well ID
3,457,940 s_7_1_Indx10 GM11995 (wellID G7) B3 0.864 10.80 12.49 B3 B3
4,062,805 s_7_1_Indx11 GM11993 (wellID F2) C3 1.016 11.09 10.92 C3 C3
3,733,084 s_7_1_Indx12 **GM10861 (wellID A9) D3 0.933 11.46 12.27 D3 D3
3,663,235 s_7_1_Indx13 GM11992 (wellID C2 ) E3 0.916 10.32 11.27 E3 E3
2,928,739 s_7_1_Indx14 GM11994 (wellID G9 ) F3 0.732 22.00 30.05 F3 F3
7,213,122 s_7_1_Indx15 GM10860 (wellID F10) G3 1.803 10.99 6.09 G3 G3
2,853,891 s_7_1_Indx16 GM12813 (wellID E8) H3 0.713 6.98 9.79 H3 H3
3,678,983 s_7_1_Indx17 GM12802 (wellID E7) A5 0.920 7.88 8.56 A5 A5
3,067,241 s_7_1_Indx18 GM12815 (wellID G12) B5 0.767 7.48 9.75 B5 B5
3,763,585 s_7_1_Indx19 GM12812 (wellID F11) C5 0.941 10.20 10.84 C5 C5
3,246,362 s_7_1_Indx20 GM12814 (wellID A7) D5 0.812 6.53 8.05 D5 D5
3,833,426 s_7_1_Indx21 GM12801 (wellID D9) E5 0.958 7.08 7.39 E5 E5
3,177,943 s_7_1_Indx22 NA12156 (wellID B3)-1 F5 0.794 7.43 9.35 F5 F5
3,349,096 s_7_1_Indx23 NA12156 (wellID B3)-2 G5 0.837 6.45 7.70 G5 G5
3,604,801 s_7_1_Indx24 NA12156 (wellID B3)-3 H5 0.901 6.49 7.21 H5 H5
4,379,684 s_7_1_Indx25 NA12156 (wellID B3)-4 A7 1.095 8.51 7.77 A7 A7
3,557,582 s_7_1_Indx26 NA12156 (wellID B3)-5 B7 0.889 10.12 11.38 B7 B7
4,331,433 s_7_1_Indx27 NA12156 (wellID B3)-6 C7 1.083 7.49 6.92 C7 C7
2,945,843 s_7_1_Indx28 NA12156 (wellID B3)-7 D7 0.736 7.62 10.34 D7 D7
3,444,795 s_7_1_Indx29 NA12156 (wellID B3)-8 E7 0.861 9.90 11.50 E7 E7
3,429,469 s_7_1_Indx30 NA12156 (wellID B3)-9 F7 0.857 7.39 8.62 F7 F7
6,808,403 s_7_1_Indx41 **PGP1iPS A11 1.702 14.16 8.32 A11 A11
5,281,331 s_7_1_Indx42 PGP1iPS-IMPRINT B11 1.320 17.42 13.19 B11 B11