Rui:SingelCell RNAseq: Difference between revisions
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>Jdeng m (→Nextera kit) |
>Jdeng m (→PCR note) |
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*[[Rui:IVT note|IVT note]] | *[[Rui:IVT note|IVT note]] | ||
*[[Rui:HRCA note|HRCA note]] | *[[Rui:HRCA note|HRCA note]] | ||
===IVT note=== | ===IVT note=== |
Revision as of 19:14, 14 November 2012
Background
- Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
- Review papers:
- Key papers:
- Single cell and cell lysis
- 1st cDNA synthesis and free primer removal
- 2nd cDNA synthesis: A-tailing vs random
- Amplification: PCR or IVT
- QC and clone
Protocols
- PCR based protocol
- IVT based protocol
- HRCA based protocol
- Nextera based protocol
- totoRNAseq protocol
QuickNotes
IVT note
IVT_based | description | start | result |
1st try | 3 sets | 2.8.11 | 2.9.11 |
Following | purification? | 2.10.11 | |
primer check | 2.15.11 | ||
clone | 2.17.11 | 2.24.11 | |
clone troubleshoot | 2.28.11 | ||
2nd round IVT | 3.2.11 | 3.3.11 | |
trouble shooting | 3.3.11 | 3.4.11 | |
Atailed_ds cDNA IVT | 3.2.11 | 3.3.11 | |
2nd try | repeat kit | 3.8.11 | 3.9.11 |
3.10.11 | 3.11.11 | ||
Atailed_ds cDNA IVT | 4.5.11 | 4.6.11 |
HRCA note
HRCA | description | start | result |
1st try | P1 | 2.3.11 | 2.4.11 |
2012
<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>
2011
<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>