Rui:SingelCell RNAseq: Difference between revisions

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==SCAP project==
==SCAP project==
* Main link [http://genome-tech.ucsd.edu/SCAP/index.php/Main_Page]
* Main link [http://genome-tech.ucsd.edu/SCAP/index.php/Main_Page]
* Libraries
* Libraries [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:RNAseq-libraries]
* Data: genome-miner.ucsd.edu:/media/Ext4T/KZ_Ext4T/RNAseq/


==Background==
==Background==

Revision as of 06:34, 9 March 2013

SCAP project

  • Main link [1]
  • Libraries [2]
  • Data: genome-miner.ucsd.edu:/media/Ext4T/KZ_Ext4T/RNAseq/

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>