Rui:SingelCell RNAseq: Difference between revisions

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*[[Rui:HRCA note|HRCA note]]
*[[Rui:HRCA note|HRCA note]]
*[[Rui:10.10.13 discussion|10.10.13 discussion]]
*[[Rui:10.10.13 discussion|10.10.13 discussion]]
==2014==
<calendar>
name=Rui:LabNotes/SingleCell
format=%name/%year-%month-%day
date=2014/10/1
view=oneyear
</calendar>


==2013==
==2013==

Revision as of 17:49, 2 April 2014

SCAP project

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

2014

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2014/10/1 view=oneyear </calendar>


2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>