Rui:SingelCell RNAseq: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>RuiLiu
mNo edit summary
>RuiLiu
mNo edit summary
Line 30: Line 30:
*[[Rui:HRCA note|HRCA note]]
*[[Rui:HRCA note|HRCA note]]
*[[Rui:10.10.13 discussion|10.10.13 discussion]]
*[[Rui:10.10.13 discussion|10.10.13 discussion]]


==2014==
==2014==

Revision as of 23:21, 17 September 2014

SCAP project

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes


2014

  • Reference RNA data analysis:
  • The first 1000 single-nucleus data workflow: 7.3; 7.4; 7.14
  • The pipeline:

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2014/10/1 view=oneyear </calendar>

2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>