Rui:SingelCell RNAseq: Difference between revisions

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==2014==
==2014==
* Reference RNA data analysis:  
* Reference RNA data analysis:  
* The first 1000 single-nucleus data workflow: 7.3; 7.4; 7.14
* The first 1000 single-nucleus data workflow: 7.3; 7.4; 7.14; 8.7
* The bulk sample analysis:
* The pipeline:
* The pipeline:



Revision as of 06:09, 13 October 2014

SCAP project

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes


2014

  • Reference RNA data analysis:
  • The first 1000 single-nucleus data workflow: 7.3; 7.4; 7.14; 8.7
  • The bulk sample analysis:
  • The pipeline:

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2014/10/1 view=oneyear </calendar>

2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>