Rui:DNAseq-Collaborations: Difference between revisions
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==Samples== | ==Samples== | ||
===Table for samples=== | |||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''hES cells''' | |||
| align="center" style="background:#f0f0f0;"|'''dFib''' | |||
| align="center" style="background:#f0f0f0;"|'''dFib-GFP colonies''' | |||
| align="center" style="background:#f0f0f0;"|'''Notes''' | |||
| align="center" style="background:#f0f0f0;"|'''P2''' | |||
| align="center" style="background:#f0f0f0;"|'''P3''' | |||
| align="center" style="background:#f0f0f0;"|'''P15''' | |||
| align="center" style="background:#f0f0f0;"|'''P30''' | |||
|- | |||
| gDNA||gDNA||A (A1, A2, A3)||A1, A2 protocol test|||||||| | |||
|- | |||
| 100c (X3)||100c (X3)||B (B1, B2, B3)||B2, B3 -> DNAseq||single colony||single colony||single colony (X3)||gDNA | |||
|- | |||
| 1000c (X4)||1000c (X4)||C (C1, C2, C3)|||||||||| | |||
|- | |||
| ||||D (D2, D3, D4)||D1 is missing|||||||| | |||
|- | |||
| ||||E |||||||||| | |||
|- | |||
| ||||F (F1, F2, F3)||F1 is missing|||||||| | |||
|- | |||
| ||||G||G is missing|||||||| | |||
|} | |||
===Dr. Juan Carlos Izpisúa Belmonte lab=== | ===Dr. Juan Carlos Izpisúa Belmonte lab=== |
Revision as of 00:12, 25 April 2012
Samples
Table for samples
hES cells | dFib | dFib-GFP colonies | Notes | P2 | P3 | P15 | P30 |
gDNA | gDNA | A (A1, A2, A3) | A1, A2 protocol test | ||||
100c (X3) | 100c (X3) | B (B1, B2, B3) | B2, B3 -> DNAseq | single colony | single colony | single colony (X3) | gDNA |
1000c (X4) | 1000c (X4) | C (C1, C2, C3) | |||||
D (D2, D3, D4) | D1 is missing | ||||||
E | |||||||
F (F1, F2, F3) | F1 is missing | ||||||
G | G is missing |
Dr. Juan Carlos Izpisúa Belmonte lab
- May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
- Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
- Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
- March 16th 2012: all colony/clone "B", Athanasia box
P2 a single marked colony P3 a single marked colony P15 a single marked colony (X3) P30 half of a dense 6-well plate (X2)
Bulk DNA isolation
- Promega wizard genomic DNA isolation kit
Quick note
2012
<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>
2011
<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>