Rui:DNAseq-Collaborations: Difference between revisions

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==Samples==
==Samples==
===Table for samples===
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''hES cells'''
| align="center" style="background:#f0f0f0;"|'''dFib'''
| align="center" style="background:#f0f0f0;"|'''dFib-GFP colonies'''
| align="center" style="background:#f0f0f0;"|'''Notes'''
| align="center" style="background:#f0f0f0;"|'''P2'''
| align="center" style="background:#f0f0f0;"|'''P3'''
| align="center" style="background:#f0f0f0;"|'''P15'''
| align="center" style="background:#f0f0f0;"|'''P30'''
|-
| gDNA||gDNA||A (A1, A2, A3)||A1, A2 protocol test||||||||
|-
| 100c (X3)||100c (X3)||B (B1, B2, B3)||B2, B3 -> DNAseq||single colony||single colony||single colony (X3)||gDNA
|-
| 1000c (X4)||1000c (X4)||C (C1, C2, C3)||||||||||
|-
| ||||D (D2, D3, D4)||D1 is missing||||||||
|-
| ||||E ||||||||||
|-
| ||||F (F1, F2, F3)||F1 is missing||||||||
|-
| ||||G||G is missing||||||||
|}


===Dr. Juan Carlos Izpisúa Belmonte lab===
===Dr. Juan Carlos Izpisúa Belmonte lab===

Revision as of 00:12, 25 April 2012

Samples

Table for samples

hES cells dFib dFib-GFP colonies Notes P2 P3 P15 P30
gDNA gDNA A (A1, A2, A3) A1, A2 protocol test
100c (X3) 100c (X3) B (B1, B2, B3) B2, B3 -> DNAseq single colony single colony single colony (X3) gDNA
1000c (X4) 1000c (X4) C (C1, C2, C3)
D (D2, D3, D4) D1 is missing
E
F (F1, F2, F3) F1 is missing
G G is missing


Dr. Juan Carlos Izpisúa Belmonte lab

  • May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
  • Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
  • Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
  • March 16th 2012: all colony/clone "B", Athanasia box
P2 a single marked colony
P3 a single marked colony
P15 a single marked colony (X3)
P30 half of a dense 6-well plate (X2)

Bulk DNA isolation

  • Promega wizard genomic DNA isolation kit

Quick note

2012

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>