Rui:DNAseq-Collaborations: Difference between revisions
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{| {{table}} border=1 | {| {{table}} border=1 | ||
| align="center" style="background:#f0f0f0;"|'''hES cells''' | | align="center" style="background:#f0f0f0;"|'''hES cells''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''dFb''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''dFb GFP colonies''' | ||
| align="center" style="background:#f0f0f0;"|'''Notes''' | | align="center" style="background:#f0f0f0;"|'''P0 Notes''' | ||
| align="center" style="background:#f0f0f0;"|'''P1''' | |||
| align="center" style="background:#f0f0f0;"|'''P2''' | | align="center" style="background:#f0f0f0;"|'''P2''' | ||
| align="center" style="background:#f0f0f0;"|'''P3''' | | align="center" style="background:#f0f0f0;"|'''P3''' | ||
| align="center" style="background:#f0f0f0;"|'''P5''' | |||
| align="center" style="background:#f0f0f0;"|'''P15''' | | align="center" style="background:#f0f0f0;"|'''P15''' | ||
| align="center" style="background:#f0f0f0;"|'''P30''' | | align="center" style="background:#f0f0f0;"|'''P30''' | ||
|- | |- | ||
| gDNA||gDNA||A (A1, A2, A3)||A1, A2 protocol test|||||||| | | gDNA||gDNA||A (A1, A2, A3)||A1, A2 protocol test||||single colony||single colony||||single colony|| | ||
|- | |- | ||
| 100c (X3)||100c (X3)||B (B1, B2, B3)||B2, B3 -> DNAseq||single colony||single colony||single colony (X3)||gDNA | | 100c (X3)||100c (X3)||B (B1, B2, B3)||B2, B3 -> DNAseq||||single colony||single colony||||single colony (X3)||gDNA | ||
|- | |- | ||
| 1000c (X4)||1000c (X4)||C (C1, C2, C3)|||||||||| | | 1000c (X4)||1000c (X4)||C (C1, C2, C3)||||||single colony||single colony|||||| | ||
|- | |- | ||
| ||||D (D2, D3, D4)||D1 is missing|||||||| | | ||||D (D2, D3, D4)||D1 is missing||single colony||single colony||||single well||single colony (X1) single well (X1)|| | ||
|- | |- | ||
| ||||E |||||||||| | | ||||E |||||||||||||| | ||
|- | |- | ||
| ||||F (F1, F2, F3)||F1 is missing|||||||| | | ||||F (F1, F2, F3)||F1 is missing||single colony||single colony||||single colony (X1) single well (X1)||single colony (X1) single well (X1)|| | ||
|- | |- | ||
| ||||G||G is missing|||||||| | | ||||G||G is missing|||||||||||| | ||
|- | |||
| |||||||||||||||||| | |||
|- | |||
| ||||HUVEC colony||P0 notes||P1||P2||P3||P5||P10||P15 | |||
|- | |||
| ||HUVEC||D (D1, D2)||||||single colony||||single colony||single colony (X2) single well (X2)||single colony (X2) single well (X2) | |||
|} | |} | ||
===Dr. Juan Carlos Izpisúa Belmonte lab=== | ===Dr. Juan Carlos Izpisúa Belmonte lab=== |
Revision as of 17:37, 26 April 2012
Samples
Table for samples
hES cells | dFb | dFb GFP colonies | P0 Notes | P1 | P2 | P3 | P5 | P15 | P30 |
gDNA | gDNA | A (A1, A2, A3) | A1, A2 protocol test | single colony | single colony | single colony | |||
100c (X3) | 100c (X3) | B (B1, B2, B3) | B2, B3 -> DNAseq | single colony | single colony | single colony (X3) | gDNA | ||
1000c (X4) | 1000c (X4) | C (C1, C2, C3) | single colony | single colony | |||||
D (D2, D3, D4) | D1 is missing | single colony | single colony | single well | single colony (X1) single well (X1) | ||||
E | |||||||||
F (F1, F2, F3) | F1 is missing | single colony | single colony | single colony (X1) single well (X1) | single colony (X1) single well (X1) | ||||
G | G is missing | ||||||||
HUVEC colony | P0 notes | P1 | P2 | P3 | P5 | P10 | P15 | ||
HUVEC | D (D1, D2) | single colony | single colony | single colony (X2) single well (X2) | single colony (X2) single well (X2) |
Dr. Juan Carlos Izpisúa Belmonte lab
- May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
- Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
- Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
- March 16th 2012: all colony/clone "B", Athanasia box
P2 a single marked colony P3 a single marked colony P15 a single marked colony (X3) P30 half of a dense 6-well plate (X2)
Bulk DNA isolation
- Promega wizard genomic DNA isolation kit
Quick note
2012
<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>
2011
<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>