Rui:DNAseq-Collaborations: Difference between revisions

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==Samples==
==Samples==
===Table for samples from A.P (Belmonte lab)===
===Dr. Juan Carlos Izpisúa Belmonte lab===
* Alice Belmonte lab box (-80)
* A.P. box (-80)
* A.P. for Rui/Kun Zhang box (-80)
 
May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
March 16th 2012: all colony/clone "B", Athanasia box
  P2 a single marked colony
  P3 a single marked colony
  P15 a single marked colony (X3)
  P30 half of a dense 6-well plate (X2)
 
====Table for samples from A.P (Belmonte lab)====
{| {{table}} border=1
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''hES cells'''
| align="center" style="background:#f0f0f0;"|'''hES cells'''
Line 34: Line 48:
|}
|}


===Dr. Juan Carlos Izpisúa Belmonte lab===
===Dr. George Church lab===
* May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
* PGP1 BAC library: -80C, Rui BAC (PGP1) box
* Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
* Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
* March 16th 2012: all colony/clone "B", Athanasia box
P2 a single marked colony
P3 a single marked colony
P15 a single marked colony (X3)
P30 half of a dense 6-well plate (X2)


===Bulk DNA isolation===
===Bulk DNA isolation===

Latest revision as of 19:47, 17 May 2012

Samples[edit]

Dr. Juan Carlos Izpisúa Belmonte lab[edit]

  • Alice Belmonte lab box (-80)
  • A.P. box (-80)
  • A.P. for Rui/Kun Zhang box (-80)
May 9th 2011: Athanasia iPS colonies samples in "Belmonte lab" box at -80C
Fibroblast samples: 2 tubes received on 11.10.11 (50k cell in 1 tube; 210k cell in 1 tube) in "collaboration" box at -80C
Dec 12th 2011: ES, Fibroblast samples: received on 12.12.11 in "Belmonte" box at -80C
March 16th 2012: all colony/clone "B", Athanasia box
 P2 a single marked colony
 P3 a single marked colony
 P15 a single marked colony (X3)
 P30 half of a dense 6-well plate (X2)

Table for samples from A.P (Belmonte lab)[edit]

hES cells dFb dFb GFP colonies P0 Notes P1 P2 P3 P5 P15 P30
gDNA gDNA A (A1, A2, A3) A1, A2 protocol test single colony single colony single colony
100c (X3) 100c (X3) B (B1, B2, B3) B2, B3 -> DNAseq single colony single colony single colony (X3) gDNA
1000c (X4) 1000c (X4) C (C1, C2, C3) single colony single colony
D (D2, D3, D4) D1 is missing single colony single colony single well single colony (X1) single well (X1)
E
F (F1, F2, F3) F1 is missing single colony single colony single colony (X1) single well (X1) single colony (X1) single well (X1)
G G is missing
HUVEC colony P0 notes P1 P2 P3 P5 P10 P15
HUVEC D (D1, D2) single colony single colony single colony (X2) single well (X2) single colony (X2) single well (X2)

Dr. George Church lab[edit]

  • PGP1 BAC library: -80C, Rui BAC (PGP1) box

Bulk DNA isolation[edit]

  • Promega wizard genomic DNA isolation kit

Quick note[edit]

2012[edit]

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011[edit]

<calendar> name=Rui:LabNotes/DNA_Collaborations format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>