Noi/NOTES/2011-12-25: Difference between revisions

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* Note:  
* Note:  
** In sequencing sample spreadsheet, these libraries were recored in lane 1-3, but they were actually loaded in lane 5-7.
** In sequencing sample spreadsheet, these libraries were recored in lane 1-3, but they were actually loaded in lane 5-7.
** Libraries from two families from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma). Also, one of UCLA sample (Indx94) was included in these libraries.
** Libraries from two families (1362 and 1454) from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma). Also, one of UCLA sample (Indx94) was included in these libraries.
* '''NP-BSPP-Hapmap-Nov15''' library normalization: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-11-15]]
* '''NP-BSPP-Hapmap-Nov15''' library normalization: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-11-15]]
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Revision as of 00:32, 26 December 2011

HL111 (NP-BSPP-Hapmap-Nov15) mapping

  • Note:
    • In sequencing sample spreadsheet, these libraries were recored in lane 1-3, but they were actually loaded in lane 5-7.
    • Libraries from two families (1362 and 1454) from HAPMAP plate, the 9 replicates for probe normalization and PGP1iPS treated with different bisulfite conversion kits (Zymoresearch and Imprint from Sigma). Also, one of UCLA sample (Indx94) was included in these libraries.
  • NP-BSPP-Hapmap-Nov15 library normalization: [[1]]
Indx Sample ID
Indx10 GM11995 (wellID G7)
Indx11 GM11993 (wellID F2)
Indx12 **GM10861 (wellID A9)
Indx13 GM11992 (wellID C2 )
Indx14 GM11994 (wellID G9 )
Indx15 GM10860 (wellID F10)
Indx16 GM12813 (wellID E8)
Indx17 GM12802 (wellID E7)
Indx18 GM12815 (wellID G12)
Indx19 GM12812 (wellID F11)
Indx20 GM12814 (wellID A7)
Indx21 GM12801 (wellID D9)
Indx22 NA12156 (wellID B3)-1
Indx23 NA12156 (wellID B3)-2
Indx24 NA12156 (wellID B3)-3
Indx25 NA12156 (wellID B3)-4
Indx26 NA12156 (wellID B3)-5
Indx27 NA12156 (wellID B3)-6
Indx28 NA12156 (wellID B3)-7
Indx29 NA12156 (wellID B3)-8
Indx30 NA12156 (wellID B3)-9
Indx41 **PGP1iPS
Indx42 PGP1iPS-IMPRINT
Indx94 UCLA-RO_Indx94
  • Sequencing data is in genome-miner: /home/kunzhang/FreshReads/111209_SN1001

Merge and trim 27bp from 5' end

less s_5_1_Indx10.txt s_5_2_Indx10.txt s_6_1_Indx10.txt s_6_2_Indx10.txt s_7_1_Indx10.txt s_7_2_Indx10.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM11995-1362.fastq
less s_5_1_Indx11.txt s_5_2_Indx11.txt s_6_1_Indx11.txt s_6_2_Indx11.txt s_7_1_Indx11.txt s_7_2_Indx11.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM11993-1362.fastq
less s_5_1_Indx12.txt s_5_2_Indx12.txt s_6_1_Indx12.txt s_6_2_Indx12.txt s_7_1_Indx12.txt s_7_2_Indx12.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM10861-1362.fastq
less s_5_1_Indx13.txt s_5_2_Indx13.txt s_6_1_Indx13.txt s_6_2_Indx13.txt s_7_1_Indx13.txt s_7_2_Indx13.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM11992-1362.fastq
less s_5_1_Indx14.txt s_5_2_Indx14.txt s_6_1_Indx14.txt s_6_2_Indx14.txt s_7_1_Indx14.txt s_7_2_Indx14.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM11994-1362.fastq
less s_5_1_Indx15.txt s_5_2_Indx15.txt s_6_1_Indx15.txt s_6_2_Indx15.txt s_7_1_Indx15.txt s_7_2_Indx15.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM10860-1362.fastq
less s_5_1_Indx16.txt s_5_2_Indx16.txt s_6_1_Indx16.txt s_6_2_Indx16.txt s_7_1_Indx16.txt s_7_2_Indx16.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12813-1454.fastq
less s_5_1_Indx17.txt s_5_2_Indx17.txt s_6_1_Indx17.txt s_6_2_Indx17.txt s_7_1_Indx17.txt s_7_2_Indx17.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12802-1454.fastq
less s_5_1_Indx18.txt s_5_2_Indx18.txt s_6_1_Indx18.txt s_6_2_Indx18.txt s_7_1_Indx18.txt s_7_2_Indx18.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12815-1454.fastq
less s_5_1_Indx19.txt s_5_2_Indx19.txt s_6_1_Indx19.txt s_6_2_Indx19.txt s_7_1_Indx19.txt s_7_2_Indx19.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12812-1454.fastq
less s_5_1_Indx20.txt s_5_2_Indx20.txt s_6_1_Indx20.txt s_6_2_Indx20.txt s_7_1_Indx20.txt s_7_2_Indx20.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12814-1454.fastq
less s_5_1_Indx21.txt s_5_2_Indx21.txt s_6_1_Indx21.txt s_6_2_Indx21.txt s_7_1_Indx21.txt s_7_2_Indx21.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GM12801-1454.fastq
less s_5_1_Indx22.txt s_5_2_Indx22.txt s_6_1_Indx22.txt s_6_2_Indx22.txt s_7_1_Indx22.txt s_7_2_Indx22.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_1.fastq
less s_5_1_Indx23.txt s_5_2_Indx23.txt s_6_1_Indx23.txt s_6_2_Indx23.txt s_7_1_Indx23.txt s_7_2_Indx23.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_2.fastq
less s_5_1_Indx24.txt s_5_2_Indx24.txt s_6_1_Indx24.txt s_6_2_Indx24.txt s_7_1_Indx24.txt s_7_2_Indx24.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_3.fastq
less s_5_1_Indx25.txt s_5_2_Indx25.txt s_6_1_Indx25.txt s_6_2_Indx25.txt s_7_1_Indx25.txt s_7_2_Indx25.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_4.fastq
less s_5_1_Indx26.txt s_5_2_Indx26.txt s_6_1_Indx26.txt s_6_2_Indx26.txt s_7_1_Indx26.txt s_7_2_Indx26.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_5.fastq
less s_5_1_Indx27.txt s_5_2_Indx27.txt s_6_1_Indx27.txt s_6_2_Indx27.txt s_7_1_Indx27.txt s_7_2_Indx27.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_6.fastq
less s_5_1_Indx28.txt s_5_2_Indx28.txt s_6_1_Indx28.txt s_6_2_Indx28.txt s_7_1_Indx28.txt s_7_2_Indx28.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_7.fastq
less s_5_1_Indx29.txt s_5_2_Indx29.txt s_6_1_Indx29.txt s_6_2_Indx29.txt s_7_1_Indx29.txt s_7_2_Indx29.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_8.fastq
less s_5_1_Indx30.txt s_5_2_Indx30.txt s_6_1_Indx30.txt s_6_2_Indx30.txt s_7_1_Indx30.txt s_7_2_Indx30.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/NA12156-1408_9.fastq
less s_5_1_Indx41.txt s_5_2_Indx41.txt s_6_1_Indx41.txt s_6_2_Indx41.txt s_7_1_Indx41.txt s_7_2_Indx41.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/PGP1iPS-ZYMO.fastq
less s_5_1_Indx42.txt s_5_2_Indx42.txt s_6_1_Indx42.txt s_6_2_Indx42.txt s_7_1_Indx42.txt s_7_2_Indx42.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/PGP1iPS-IMPRINT.fastq
less s_5_1_Indx94.txt s_5_2_Indx94.txt s_6_1_Indx94.txt s_6_2_Indx94.txt s_7_1_Indx94.txt s_7_2_Indx94.txt | /home/kunzhang/bin/trimFastq.pl 27 83 >/home/nplongth/Noi_scratch/HL111_BSPP-Hapmap_20111224/GK0281-004.fastq