Noi/NOTES/2012-2-29: Difference between revisions

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'''Link to calendar:'''[[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes]]
= ASM analysis of WGBB12878 =
= ASM analysis of WGBB12878 =
* Directory: '''/media/SeqStore2/120209_HiSeq/HL_WGBS/'''
* Directory: '''/media/SeqStore2/120209_HiSeq/HL_WGBS/'''

Revision as of 02:17, 12 March 2012

Link to calendar:[[1]]

ASM analysis of WGBB12878

  • Directory: /media/SeqStore2/120209_HiSeq/HL_WGBS/
s_7_1_sequence.txt.gz
s_7_2_sequence.txt.gz
s_8_1_sequence.txt.gz
s_8_2_sequence.txt.gz
  • Concatenate and uncompressed data (no trimming required)
nohup less /media/SeqStore2/120209_HiSeq/HL_WGBS/*.gz > 12878.fastq
  • In Master Execution Script, PipelineExecute-NP.pl
my $root = "/home/expresso/Noi_ASM/WGBS_12878/";
my $readlength = 100;
  • Note: After finishing concatenate sequencing data into 12878.fastq, I got only 23.9 Gb data. I repeated again, but got the same amount of data, I guess that it possible that the space on expresso is not enough to put the whole data.