Brandon:Protocols/taq amplification2: Difference between revisions

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==taq amplification==
==Standard PCR cycling methods for different enzymes/procedures==
 
 
*KAPA SYBR
 
12.5 uL KAPA SYBR
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template
KAPA SYBR cycles:
98C 3min, (98C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
 
 
*taq amplification


used taq2x for amplification, X35 cycles
used taq2x for amplification, X35 cycles
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  taq2x cycles:
  taq2x cycles:
  95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
  95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
*KAPA2G Robust

Revision as of 00:41, 18 May 2012

Standard PCR cycling methods for different enzymes/procedures

  • KAPA SYBR
12.5 uL KAPA SYBR
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template

KAPA SYBR cycles:
98C 3min, (98C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever


  • taq amplification

used taq2x for amplification, X35 cycles

12.5 uL taq2X
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template

taq2x cycles:
95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever


  • KAPA2G Robust