Matthew Cai: Difference between revisions
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*[[Matt:LabNotes/Bead Purification Protocol|Bead Purification Protocol]] | *[[Matt:LabNotes/Bead Purification Protocol|Bead Purification Protocol]] | ||
*[[Matt:LabNotes/Qubit Protocol|Qubit Protocol]] | *[[Matt:LabNotes/Qubit Protocol|Qubit Protocol]] | ||
*[[Matt:LabNotes/2014-2-19|BF "Skeleton" Image]] | |||
==Sequencing Runs== | ==Sequencing Runs== |
Revision as of 23:00, 20 March 2014
Projects
in Situ Sequencing
- Padlock Probe Design
- Probe Production and Capture
- CA12k Capture (MiSeq_130325) Analysis
- CA12k End Sequencing (HL152_130524) Analysis
- Agi26k End Sequencing (HL155_130628) Analysis
- Quantifying Errors in CA12k and Agi26k Oligo Pools
Notebook
2013
<calendar> name=Matt format=%name:LabNotes/%year-%month-%day date=2013/01/01 view=oneyear </calendar>
2014
<calendar> name=Matt format=%name:LabNotes/%year-%month-%day date=2014/01/01 view=oneyear </calendar>
Protocols
Sequencing Runs
130325_MiSeq: CA12k capture of gDNA, cDNAwRNase, and cDNA-RNase
130524_HL152 (Lane 2, unassigned): CA12k oligos
130628_HL155 (Lane 3, Indx 10 & 12): Agi26k_0gap and Agi26k_20gap oligos
130729_MiSeq: Agi26k_0gap and Agi26k_20gap capture of gDNA and cDNA
Probe Sets
CA12k: 12,355 probes (170nt)
Agi26k_0gap: 12,964 probes (193nt)
Agi26k_20gap: 13,179 probes (193nt)