Preparation of Matrigel plates: Difference between revisions
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>TinaLo (Created page with "'''Preparation of Matrigel plates''' 1. Thaw a 145 ul aliquot of Matrigel at 4°C for 1-2 h before use. 2. Add 12.5 ml of cold DMEM/F12 (4°C) to the Matrigel solution tubes...") |
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1. Thaw a 145 ul aliquot of Matrigel at 4°C for 1-2 h before use. | 1. Thaw a 145 ul aliquot of Matrigel at 4°C for 1-2 h before use. | ||
Latest revision as of 00:33, 2 June 2012
1. Thaw a 145 ul aliquot of Matrigel at 4°C for 1-2 h before use.
2. Add 12.5 ml of cold DMEM/F12 (4°C) to the Matrigel solution tubes. Pipet gently to avoid bubbles formation.
3. Add 1 ml of the Matrigel working solution to a 35 mm culture dish and rock gently to distribute the solution evenly.
4. Leave the plate at RT for 1-2 h or put it at 4°C overnight.
- Matrigel plates are good for 1 weeks at 4°C, although fresh plates are preferable.