Tina:LabNotes/2012-5-25: Difference between revisions
Jump to navigation
Jump to search
>TinaLo mNo edit summary |
>TinaLo mNo edit summary |
||
Line 1: | Line 1: | ||
*Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) | |||
*Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80. | |||
*[[Preparation of 0.1% gelatin solution]] | |||
*[[Preparation of gelatin coated plates]] | |||
*Seed MEF on gelatin coating plates (using [[MEF culture medium]]) |
Revision as of 22:54, 4 June 2012
- Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well)
- Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80.
- Preparation of 0.1% gelatin solution
- Preparation of gelatin coated plates
- Seed MEF on gelatin coating plates (using MEF culture medium)