Calendar/2012-7-13: Difference between revisions

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>Alan6017518
(Created page with "==Amplification of Dr. Yi Zhang's DiP libraries Sample== ==qPCR with N2 Barcodes== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXC...")
 
>Alan6017518
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==qPCR with N2 Barcodes==
==qPCR with N2 Barcodes==
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
|- style="background-color:#FCD5B4;font-size:8pt;font-weight:bold" align="center"
|style="text-decoration:underline" width="76" height="15" | DIP Sample
|style="text-decoration:underline" width="76" height="15" | DIP Sample
| width="65" | N2 Barcode
| width="65" | N2 Barcode


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
|style="text-decoration:none" height="15" | shCONT-input
|style="text-decoration:none" height="15" | shCONT-input
| align="center" | 1
| align="center" | 1


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shCONT-IgG
| height="15" | shCONT-IgG
| align="center" | 2
| align="center" | 2


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shCONT-5mC
| height="15" | shCONT-5mC
| align="center" | 3
| align="center" | 3


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shCONT-5hmC
| height="15" | shCONT-5hmC
| align="center" | 4
| align="center" | 4


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shCONT-5fc
| height="15" | shCONT-5fc
| align="center" | 5
| align="center" | 5


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shCONT-5caC
| height="15" | shCONT-5caC
| align="center" | 6
| align="center" | 6


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-input
| height="15" | shTDG-input
| align="center" | 7
| align="center" | 7


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-IgG
| height="15" | shTDG-IgG
| align="center" | 8
| align="center" | 8


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-5mC
| height="15" | shTDG-5mC
| align="center" | 9
| align="center" | 9


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-5hmC
| height="15" | shTDG-5hmC
| align="center" | 10
| align="center" | 10


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-5fC
| height="15" | shTDG-5fC
| align="center" | 11
| align="center" | 11


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | shTDG-caC
| height="15" | shTDG-caC
| align="center" | 12
| align="center" | 12
Line 56: Line 56:
|}
|}
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="background-color:#B7DEE8;font-size:12pt;font-weight:bold" align="center"
|- style="background-color:#B7DEE8;font-size:8pt;font-weight:bold" align="center"
|style="text-decoration:underline" width="115" height="15" | Content
|style="text-decoration:underline" width="115" height="15" | Content
| width="110" | Volume Per Reaction
| width="110" | Volume Per Reaction
| width="98" | MM
| width="98" | MM


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
|style="text-decoration:none" height="15" | Template
|style="text-decoration:none" height="15" | Template
| align="center" | 10
| align="center" | 10
  | Do not add to MM
  | Do not add to MM


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | 5uM PCR F+R Barcode
| height="15" | 5uM PCR F+R Barcode
| align="center" | 1
| align="center" | 1
  | Do not add to MM
  | Do not add to MM


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | 50X SYBG
| height="15" | 50X SYBG
| align="center" | 0.2
| align="center" | 0.2
| align="center" | 2.64
| align="center" | 2.64


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | 2X PhusionHF MM
| height="15" | 2X PhusionHF MM
| align="center" | 25
| align="center" | 25
| align="center" | 330
| align="center" | 330


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | H20
| height="15" | H20
| align="center" | 13.8
| align="center" | 13.8
| align="center" | 182.16
| align="center" | 182.16


|- style="font-size:12pt" align="center"
|- style="font-size:8pt" align="center"
| height="15" | Total Volume
| height="15" | Total Volume
| align="center" | 50
| align="center" | 50

Revision as of 17:16, 13 July 2012

Amplification of Dr. Yi Zhang's DiP libraries Sample

qPCR with N2 Barcodes

DIP Sample N2 Barcode
shCONT-input 1
shCONT-IgG 2
shCONT-5mC 3
shCONT-5hmC 4
shCONT-5fc 5
shCONT-5caC 6
shTDG-input 7
shTDG-IgG 8
shTDG-5mC 9
shTDG-5hmC 10
shTDG-5fC 11
shTDG-caC 12
Content Volume Per Reaction MM
Template 10 Do not add to MM
5uM PCR F+R Barcode 1 Do not add to MM
50X SYBG 0.2 2.64
2X PhusionHF MM 25 330
H20 13.8 182.16
Total Volume 50 660
  • Add 20ul 10uM N2-bar-code primers into strip tube
  • Add 20ul 10uM PCR_F primers and mix
  • We have 5uM F+R N2 Ind primer (Store at 4C Alice yellow 0.2ul tube rack)
  • Add 10ul template (for low volume sample add 5ul sample and add 5ul h2o) and 1ul barcode primers (using multi channel pipette) to strip tube
  • Add 39ul MM to each well
  98C 30sec 
  (98C 10sec -> 63C 20sec-> 72C 30sec) x 20 cycles
  72C 2min  
  15C hold.