Noi/NOTES/2012-7-12: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Noi
(Created page with "= epMotion test (AMPure bead purification) = * Things need to adjust ** Speed of aspiration and dispension of PCR product ** Avoid to reuse the tips to pipette the same reagen...")
 
>Noi
(Blanked the page)
Line 1: Line 1:
= epMotion test (AMPure bead purification) =
 
* Things need to adjust
** Speed of aspiration and dispension of PCR product
** Avoid to reuse the tips to pipette the same reagent like EtOH in washing step. This need to adjust protocol to be a little more complicated in fill in the sample number in each step.
= Plan of epMotion testing (two round AMPure bead purification and vary amount of start DNA amount =
* DNA amount range (in 80ul of the liquid)
** 5ng
** 10ng
** 25ng
** 50ng
** 100ng
** 200ng
* The pooled PCR product from Sergio's samples is approximately 22ng/ul by PAGE quantification
{| {{table}} border =1
| align="center" style="background:#f0f0f0;"|'''DNA amount (ng)'''
| align="center" style="background:#f0f0f0;"|'''Total volume (ul)'''
| align="center" style="background:#f0f0f0;"|'''Conc (ng/ul)'''
| align="center" style="background:#f0f0f0;"|'''DNA (stock 22ng/ul)'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
| align="center" style="background:#f0f0f0;"|'''Total volume'''
| align="center" style="background:#f0f0f0;"|'''DNA amount (ng)'''
|-
| 5||80||0.0625||2.84||997.16||1000.00||5
|-
| 10||80||0.1250||5.68||994.32||1000.00||10
|-
| 25||80||0.3125||14.20||985.80||1000.00||25
|-
| 50||80||0.6250||28.41||971.59||1000.00||50
|-
| 100||80||1.2500||56.82||943.18||1000.00||100
|-
| 200||80||2.5000||113.64||886.36||1000.00||200
|}

Revision as of 02:04, 14 July 2012